J Korean Assoc Pediatr Surg.  2002 Dec;8(2):101-106.

Inhibition of Tumor Growth and Angiogenesis by KJ3, Betulinic Acid, and Fumagillin in Mouse Neuroblastoma

• Choi SH
• Lee JH
• Hwang EH

Abstract

The antiangiogenic effects of novel agent KJ3, Betulinic acid, and Fumagillin on the neovascularization were studied by examining ultrastructural alterations in the vasculature of synthetic gelform and mouse neuroblastoma C1300. Small pieces of gelform with 0.4 % agar were introduced subcutaneously (s.c.) in 7 week old male CH3/HeJ mice. After the LD50s were determined by FACS analysis, a third of LD50 of three drugs were injected either locally or intraperitoneally every other day for 14 days. A/J mice were inoculated s.c. with the C1300 neuroblastoma cell line, then either saline or three drugs were injected in the same manner. The antiangiogenic effects of three drugs were studied by measuring the histologic changes in tumors, and immunostaining for CD34, VIII/vWF, CD105, and thymidine phosphorylase. In the drug treated groups, the number of vessels in gelform experiments and C1300 neuroblastoma experiments were lower than the corresponding values in the control. The histologic findings were significantly different in drug treated groups on day 7, but these were not significant on day 14. These results imply that antiangiogenic agents were effective when the tumor burden is minimal.

Keyword

KJ3; Betulinic acid; Fumagillin; Antiangiogenesis; Neuroblastoma

MeSH Terms

Agar
Angiogenesis Inhibitors
Animals
Cell Line
Humans
Lethal Dose 50
Male
Mice*
Neuroblastoma*
Thymidine Phosphorylase
Tumor Burden
Agar
Angiogenesis Inhibitors
Thymidine Phosphorylase