J Lung Cancer.
2004 Mar;3(1):31-37.
Polymorphisms of DNA Repair Gene XRCC1 and Radiation Sensitivity
- Affiliations
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- 1Department of Radiation Oncology, School of Medicine, Ajou University, Suwon, Korea.
- 2Department of Radiation Oncology, Asan Medical Center, University of Ulsan College of Medicine, Seoul, Korea. ekchoi@amc.seoul.kr
- 3Department of Microbiology, College of Medicine, Inha University, Incheon, Korea.
- 4Department of Occupational and Environmental Medicine, College of Medicine, Inha University, Incheon, Korea.
- 5Department of Therapeutic Radiology, College of Medicine and Medical Research Center, Seoul National University, Seoul, Korea.
Abstract
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PURPOSE: The goal of this study was to find lung cancer-related single nucleotide polymorphisms (SNP) and define their association with clinical results. Material and Methods: One hundred and thirty-six non-small cell lung cancer patients, who received radiotherapy at the Asan Medical Center, were recruited between August 2002 and September 2003. Demographic and clinical informations were obtained from a self-administered questionnaire and from the subject's medical records, respectively. Blood samples were collected from all study subjects at the time of enrollment. Genomic DNA was extracted from peripheral blood lymphocytes using a QIAamp DNA Blood Mini Kit. TaqMan assay, denaturing HPLC and single base pair primer extension assay using SNaPshot kits were employed as the SNP screening techniques. The candidate SNP for screening was XRCC1-R399Q.
RESULTS
Patients carrying the 399Gln variant allele had a significantly longer progression-free survival than those with the 399Arg homozygote in tumor stages I-IIIa (p=0.005). In the Cox-proportional hazards model, the XRCC1 codon 399 polymorphism was a statistically significant predictor for progression-free survival in tumor stages I-IIIa (p=0.03).
CONCLUSION
The use of molecular predictors of the progression-free survival in non-small cell lung cancer patients, particularly at stages I-IIIa, may provide important criteria for prognosis of the patients undergoing radiotherapy. However, there is still a need for further study to establish the role of these polymorphisms as useful predictors
MeSH Terms
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Alleles
Base Pairing
Carcinoma, Non-Small-Cell Lung
Chromatography, High Pressure Liquid
Chungcheongnam-do
Codon
Disease-Free Survival
DNA Repair*
DNA*
Homozygote
Humans
Lung
Lung Neoplasms
Lymphocytes
Mass Screening
Medical Records
Polymorphism, Single Nucleotide
Prognosis
Proportional Hazards Models
Surveys and Questionnaires
Radiation Tolerance*
Radiotherapy
Codon
DNA
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