J Korean Soc Transplant.  2003 Dec;17(2):192-196.

Pathologic Scoring and Postoperative Liver Function in Canine Partial Liver Transplantation: after Desferrioxamine Exposure

• Chung KY
• Jeong GY
• Kim YS

Abstract

PURPOSE
Liver biopsy plays an important role in the histopathological evaluation of the transplanted liver, but till now pretransplant graft biopsy has limited role in predicting primary non function of the graft. Desferrioxamine (DFO), the iron chelating agent, has been known to be effective in reducing rat liver ischemia-reperfusion injury. We tried desferrioxamine in canine partial liver transplantation, and pathologic scores were compared.
METHODS
~70% partial liver was harvested and reimplanted in same mongrel dog weighing about 25 kg. Desferrioxamine (20 mg/kg) was infused via splenic vein just from the beginning of reperfusion of the partial liver graft (n=5). Serum aspartate aminotransferase (AST) Alkaline phosphatase (ALP), Lactate dehydrogenase (LDH) were checked and compared with the control group (n=5). Morphological liver injury score were compared to the control group. Statistical analysis was done with independent T-test.
RESULTS
Total ischemic time was 4 hours and 42 minutes in average. AST level was significantly lower in Desferrioxamine group at 1 hour and 48 hours after reperfusion, (P=0.4) ALP level was significantly lower in desferrioxamine group at 48 hours after reperfusion (P=0.4). LDH level in desferrioxamine group was lower than that of control group but without statistical significance. The pathologic score at 1 hour after reperfusion showed a reduced degree of sinusoidal injury among the DFO group but the difference was not statistically significant. The pathologic score just before harvest of the graft showed no correlation with serum AST, ALP, LDH levels at that time or at 1 hour or 48 hours after reperfusion. Only the pathologic score at 1 hour after reperfusion had significant correlation with the serum LDH levels at 48 hours after reperfusion.
CONCLUSION
In canine live donor partial liver transplantation, desferrioxamine infusion just before reperfusion might be an effective way of reducing ischemia-reperfusion injury. And the pathologic grading on samples obtained at 1 hour after reperfusion showed a significant correlation with subsequent liver function

Keyword

Canine partial liver transplantation; Graft biopsy; Desferrioxamine; Ischemia-reperfusion injury; Pathologic score

MeSH Terms

Alkaline Phosphatase
Animals
Aspartate Aminotransferases
Biopsy
Deferoxamine*
Dogs
Humans
Iron
L-Lactate Dehydrogenase
Liver Transplantation*
Liver*
Rats
Reperfusion
Reperfusion Injury
Splenic Vein
Tissue Donors
Transplants
Alkaline Phosphatase
Aspartate Aminotransferases
Deferoxamine
Iron
L-Lactate Dehydrogenase