J Korean Acad Rehabil Med.  2003 Jun;27(3):382-387.

Expression of Caveolin-3 in the Muscle Cell and Tissue

• Kwon BS
• Lee SJ
• Hyun JK
• Jun DJ
• Joo HW
• Kim BH
• Shin DH

Abstract

OBJECTIVE
Caveolae are the microdomain of the plasma membrane that have been implicated in signal transduction and caveolin is a principal component of the caveolae. Caveolin-3, a family of caveolin related protein, is expressed only in muscle tissue. Here we examined the expression of caveolin-3 in the course of myobalst differentiation and within the muscle tissue. METHOD: L6 cell, rat skeletal myoblast, was cultured in the low mitogen medium and caveolin-3 expression was observed both by immunocytochemistry and western blot analysis. Localization of caveolin-3 within the muscle tissue was investigated and compared to that of dystrophin. RESULTS: While caveolin-3 was not expressed in the proliferating myolast, caveolin-3 was expressed in the differentiated myoblast. Caveolin-3 and dystrophin were co-expressed in the membrane of muscle tissue and integrated density of caveolin-3 was elevated in the area of muscle injury. In the Duchenne muscular dystrophy, caveolin-3 was expressed in the membrane of muscle tissue, but dystrophin was not. CONCLUSION: Caveolin-3 was induced during the myobalst differentiation and its expression was increased during the muscle regeneration. Caveolin-3 was physically associated with dystrophin as a complex, but not absolutely required for the biogenesis of dystrophin complex.

Keyword

Caveolin-3; Dystrophin; Duchenne muscular dystrophy; Myoblast; Skeletal muscle

MeSH Terms

Animals
Organelle Biogenesis
Blotting, Western
Caveolae
Caveolin 3*
Cell Membrane
Dystrophin
Humans
Immunohistochemistry
Membranes
Muscle Cells*
Muscle, Skeletal
Muscular Dystrophy, Duchenne
Myoblasts
Myoblasts, Skeletal
Rats
Regeneration
Signal Transduction
Caveolin 3
Dystrophin