J Korean Acad Periodontol.  2004 Sep;34(3):489-498.

RANKL expression is mediated by p38 MAPK in rat periodontal ligament cells

• Kim CC
• Kim YJ
• Chung HJ
• Kim OS

Abstract

Recent studies have demonstrated that human periodontal ligament cells express receptor activation of nuclear factor kappaB ligand (RANKL) which enhances the bone resorbing activity of osteoclasts differentiated from hematopoietic preosteoclasts. The purpose of this study is to determine the effects of p38 MAPK and JNK kinase upon regulating RANKL and OPG in response to IL-1beta(1 ng/ml) in rat periodontal ligament cells. Soluble RANKL was measured by immunoassay. The effects of p38 MAPK on RANKL and OPG expression was determined by RT-PCR. The results were as follows: 1. Periodontal ligament cells which stimulated by IL-1beta increased soluble RANKL synthesis by dose-dependent pattern. 2. p38 MAP kinase inhibitor (SB203580) showed regulation of soluble RANKL expression by dose-dependent manners. 3. p38 MAP kinase inhibitor (SB203580) regulated the expression of RANKL, but it dose regulate the expresseion of OPG. 4. JNK (c-jun NH2-terminal kinase) inhibitor (PD98059) did not regulate mRANKL and mOPG. These results suggested that p38 MAPK play a significant role in RANKL gene expression.

Keyword

RANKL; OPG; p38 MAPK; periodontal ligament cell; RT-PCR

MeSH Terms

Animals
Gene Expression
Humans
Immunoassay
MAP Kinase Kinase 4
Osteoclasts
p38 Mitogen-Activated Protein Kinases*
Periodontal Ligament*
Rats*
MAP Kinase Kinase 4
p38 Mitogen-Activated Protein Kinases