Obstet Gynecol Sci.  2013 May;56(3):167-175.

The frequency and clinical significance of intra-amniotic inflammation defined as an elevated amniotic fluid matrix metalloproteinase-8 in patients with preterm labor and low amniotic fluid white blood cell counts

• Park CW
• Yoon BH
• Kim SM
• Park JS
• Jun JK

Abstract

OBJECTIVE
To determine the frequency and clinical significance of intra-amniotic inflammation (IAI) defined as an elevated amniotic fluid (AF) matrix metalloproteinase-8 (MMP-8) concentration in patients with preterm labor and intact membranes (PTL) and low AF white blood cell (WBC) counts.
METHODS
Adverse pregnancy outcomes were compared according to the presence or absence of IAI in 220 singleton gestations who underwent amniocentesis due to PTL (gestational age<35.7 weeks) and had low AF WBC counts (<19 cells/mm3). Adverse pregnancy outcomes included preterm birth within 5 days of amniocentesis, acute histologic chorioamnionitis (acute-HCA) and positive AF culture. IAI was defined as an elevated AF MMP-8 concentration (> or =23 ng/mL).
RESULTS
IAI was present in 19% of study population. Adverse pregnancy outcomes were significantly more frequent in patients with IAI than in those without IAI (preterm birth within 5 days of amniocentesis, 88% vs. 41%; acute-HCA, 47% vs. 11%; positive AF culture, 10% vs. 2%; each for P<0.05). Patients with IAI had a significantly shorter median amniocentesis-to-delivery interval than those without IAI (7.8 hours [0.01-3,307.3 hours] vs. 310.3 hours [0.01-2,973.8 hours]; P<0.001 from survival analysis). Multiple logistic regression analysis demonstrated that only an IAI (odds ratio, 3.3; 95% confidence interval, 1.5-7.3; P<0.005) retained a statistical significance in the prediction of acute-HCA after other confounding variables were adjusted.
CONCLUSION
Approximately one-fifth of patients with PTL and low AF WBC counts have an evidence of IAI and are at risk for impending preterm delivery and acute-HCA when AF MMP-8 concentration is used.

Keyword

Amniotic fluid; Chorioamnionitis; Inflammation; Matrix metalloproteinase-8; Preterm labor; White blood cell

MeSH Terms

Amniocentesis
Amniotic Fluid
Chorioamnionitis
Confounding Factors (Epidemiology)
Female
Humans
Inflammation
Leukocyte Count
Leukocytes
Logistic Models
Matrix Metalloproteinase 8
Membranes
Obstetric Labor, Premature
Parturition
Pregnancy
Pregnancy Outcome
Premature Birth
Matrix Metalloproteinase 8

Figure

• 

  Fig. 1 Survival analysis of amniocentesis-to-delivery interval according to the presence or absence of IAI in patients with preterm labor and low AF WBC counts (IAI (+), median 7.8 hours [range, 0.01-3,307.3 hours] vs. IAI (-), median 310.3 hours [range, 0.01-2,973.8 hours]; P<0.001). This difference remained significant after adjustment for gestational age at amniocentesis. IAI, intra-amniotic inflammation; AF, amniotic fluid; MMP-8, matrix metalloproteinase-8; WBC, white blood cell.

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  Fig. 2 Amniocentesis-to-delivery interval according to AF MMP-8 concentrations in cases with intra-amniotic inflammation (defined as AF MMP-8 concentration≥23 ng/mL) in patients with preterm labor and low AF WBC counts. AF, amniotic fluid; MMP-8, matrix metalloproteinase-8; WBC, white blood cell.

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