Nutr Res Pract.  2012 Feb;6(1):3-8.

Suppression of oxidative stress by grape seed supplementation in rats

• Choi SK
• Zhang XH
• Seo JS

Abstract

Polyphenol-rich grape seeds have a beneficial effect on human health. The present study was performed to investigate the effects of grape seeds on antioxidant activities in rats. Male Sprague-Dawley rats were randomly divided into a control diet group (C), a high-fat diet group (HF), a 5% grape seed-supplemented control diet group (G), and a 5% grape seed-supplemented high-fat diet group (HG). Dietary supplementation with grape seeds reduced serum concentrations of lipid peroxides compared with those in the C and HF groups. The hepatic level of lipid peroxides decreased significantly in the grape seed groups compared with that in the C and HF groups. Superoxide dismutase activity in the G group increased significantly compared with that in the C group. Catalase activity tended to be higher by feeding grape seeds. The grape seed diet increased glutathione peroxidase activity in the C group. Glutathione-S-transferase activity increased significantly in the G group compared with that in the C group. Hepatic content of total glutathione increased significantly in the HG group but decreased significantly in the HF group. The ratio of reduced glutathione and oxidized glutathione increased by feeding the grape seed diet. Total vitamin A concentration was significantly higher in HG group than in other groups. Liver tocopherol content of the G and HG groups was significantly higher than that of the control groups. These results suggest that dietary supplementation with grape seeds is beneficial for suppressing lipid peroxidation in high fat-fed rats.

Keyword

Grape seed; oxidative stress; antioxidant; high fat; rat

MeSH Terms

Animals
Catalase
Diet
Diet, High-Fat
Dietary Supplements
Glutathione
Glutathione Disulfide
Glutathione Peroxidase
Humans
Lipid Peroxidation
Lipid Peroxides
Liver
Male
Oxidative Stress
Rats
Rats, Sprague-Dawley
Seeds
Superoxide Dismutase
Tocopherols
Vitamin A
Vitis
Catalase
Glutathione
Glutathione Disulfide
Glutathione Peroxidase
Lipid Peroxides
Superoxide Dismutase
Tocopherols
Vitamin A

Figure

• 

  Fig. 1 Effect of grape seed supplementation on the serum concentration (A) and hepatic concentration (B) of lipid peroxides in rats fed a control or high-fat diet. Groups are the same as in Table 1. MC, microsome. Means ± SD (n = 8). Values with the same superscript letters are not significantly different at P < 0.05.

• 

  Fig. 2 Effect of grape seed supplementation on hepatic level of total glutathione (A) and liver GSH/GSSG (B) in rats fed a control or high-fat diet. Groups are the same as in Table 1. Means ± SD (n = 8). Values with the same superscript letter are not significantly different at P < 0.05.

• 

  Fig. 3 Effect of grape seed supplementation on hepatic concentrations of α-tocopherol in rats fed a control or high-fat diet. Groups are the same as in Table 1. Means ± SD (n = 8). Values with the same superscript letter are not significantly different at P < 0.05.

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