Mycobiology.
2013 Dec;41(4):234-242.
Biocontrol Activity of Bacillus amyloliquefaciens CNU114001 against Fungal Plant Diseases
- Affiliations
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- 1Laboratory of Plant Pathology, Department of Agricultural Biology, College of Agriculture and Life Sciences, Chungnam National University, Daejeon 305-764, Korea. shunyu@cnu.ac.kr
- 2Department of Plant Protection, College of Agriculture, Yangtze University, Jingzhou 434025, China.
- 3Research Center for Biobased Chemistry, Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea.
- 4Division of Biotechnology, College of Environmental and Bioresource Sciences, Chonbuk National University, Iksan 570-752, Korea.
Abstract
- A total of 62 bacterial isolates were obtained from Gomsohang mud flat, Mohang mud flat, and Jeju Island, Republic of Korea. Among them, the isolate CNU114001 showed significant antagonistic activity against pathogenic fungi by dual culture method. The isolate CNU114001 was identified as Bacillus amyloliquefaciens by morphological observation and molecular data analysis, including 16SrDNA and gyraseA (gyrA) gene sequences. Antifungal substances of the isolate were extracted and purified by silica gel column chromatography, thin layer chromatography, and high performance liquid chromatography. The heat and UV ray stable compound was identified as iturin, a lipopeptide (LP). The isolate CNU114001 showed broad spectrum activity against 12 phytopathogenic fungi by dual culture method. The semi purified compound significantly inhibits the mycelial growth of pathogenic fungi (Alternaria panax, Botrytis cinera, Colletotrichum orbiculare, Penicillium digitatum, Pyricularia grisea and Sclerotinia sclerotiorum) at 200 ppm concentration. Spore germ tube elongation of Botrytis cinerea was inhibited by culture filtrate of the isolate. Crude antifungal substance showed antagonistic activity against cucumber scleotiorum rot in laboratory, and showed antagonistic activity against tomato gray mold, cucumber, and pumpkin powdery mildew in greenhouse condition.
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