Mycobiology.  2017 Sep;45(3):213-219. 10.5941/MYCO.2017.45.3.213.

Biocontrol Activity of Volatile-Producing Bacillus megaterium and Pseudomonas protegens against Aspergillus flavus and Aflatoxin Production on Stored Rice Grains

Affiliations
  • 1Laboratory of Plant Disease and Biocontrol, Department of Biosystems and Biotechnology, Korea University, Seoul 02841, Korea. kidkim@korea.ac.kr
  • 2Institute of Life Science and Natural Resources, Korea University, Seoul 02841, Korea.

Abstract

In our previous study, three bacterial strains, Bacillus megaterium KU143, Microbacterium testaceum KU313, and Pseudomonas protegens AS15, were selected as effective biocontrol agents against Aspergillus flavus on stored rice grains. In this study, we evaluated the inhibitory effects of the volatiles produced by the strains on A. flavus growth and aflatoxin production on stored rice grains. The three strains significantly reduced mycelial growth of A. flavus in dual-culture assays compared with the negative control strain, Sphingomonas aquatilis KU408, and an untreated control. Of these tested strains, volatiles produced by B. megaterium KU143 and P. protegens AS15 markedly inhibited mycelial growth, sporulation, and conidial germination of A. flavus on agar medium and suppressed the fungal populations in rice grains. Moreover, volatiles produced by these two strains significantly reduced aflatoxin production in the rice grains by A. flavus. To our knowledge, this is the first report of the suppression of A. flavus aflatoxin production in rice grains using B. megaterium and P. protegens volatiles.

Keyword

Aflatoxin; Aspergillus flavus; Bacillus megaterium; Bacterial volatiles; Pseudomonas protegens

MeSH Terms

Aflatoxins*
Agar
Aspergillus flavus*
Aspergillus*
Bacillus megaterium*
Bacillus*
Germination
Pseudomonas*
Sphingomonas
Aflatoxins
Agar
Full Text Links
  • MB
Actions
Cited
CITED
export Copy
Close
Share
  • Twitter
  • Facebook
Similar articles
Copyright © 2024 by Korean Association of Medical Journal Editors. All rights reserved.     E-mail: koreamed@kamje.or.kr