Mycobiology.
2010 Dec;38(4):274-281.
Growth and Cultural Characteristics of Cordyceps cardinalis Collected from Korea
- Affiliations
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- 1Mushroom Research Division, National Institute of Horticultural and Herbal Science, Rural Development Administration, Suwon 441-707, Korea.
- 2Green Energy Mission/Nepal, Anam Nagar, Kathmandu P.O. Box 10647, Nepal.
- 3Division of Forest Biodiversity, Korea National Arboretum, Pocheon 487-820, Korea.
- 4Donghae Agricultural Technology Center, Donghae 240-030, Korea.
- 5Cordyceps Institute of Mushtech, Chuncheon 200-936, Korea. cordyceps@hanmail.net
Abstract
- Cordyceps cardinalis was reported in Japan and the USA in 2004, and its fruiting bodies have recently been cultured in Korea. Herbarium specimens preserved at the Cordyceps Research Institute, Mushtech, Korea were revised and identified as C. cardinalis, based on morphological characters and conidial structures. Most of the C. cardinalis specimens were collected from Mt. Halla in Jeju-do. The effects of various nutritional sources and environmental conditions such as temperature and pH on mycelial growth of C. cardinalis were studied. Oatmeal agar, Martin's peptone dextrose agar, and Schizophyllum (mushroom) genetics complete medium plus yeast extract resulted in the best mycelial growth. Among carbon sources, cereals, and nitrogen sources, maltose, oatmeal, and peptone resulted in the best mycelial growth respectively. Mineral salts helped to increase growth rate but only resulted in thin mycelial density, similar to water agar. A temperature of 25degrees C and a pH of 7 resulted in the highest mycelial growth. Based on these results, a Cordyceps cardinalis composite medium (CCM) was formulated with 1% maltose, 2% oatmeal, 1% peptone, and 2% agar. Use of the CCM resulted in slightly better mycelial growth than that of other commonly used agar media. Only organic nitrogen sources imparted a reddish pigmentation to the agar media, but this character diminished after several subcultures. A 7 day culture duration resulted in the best mycelial growth.
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