Mycobiology.
2010 Jun;38(2):133-136.
Optimum Conditions for Artificial Fruiting Body Formation of Cordyceps cardinalis
- Affiliations
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- 1Donghae Agricultural Technology Center, Gangwon 240-030, Korea.
- 2Green Energy Mission/Nepal, Anam Nagar, Kathmandu 10647, Nepal.
- 3National Museum of Nature and Science, Department of Botany, Tsukuba, Japan.
- 4Division of Forest Biodiversity, Korea National Arboretum, Pocheon 481-820, Korea.
- 5Cordyceps Institute of Kangwon National University, Department of Applied Biology, Kangwon National University, Chuncheon 200-701, Korea. cordyceps@hanmail.net
- 6Cordyceps Institute of Mushtech, Chuncheon 200-936, Korea.
Abstract
- Stromatal fruiting bodies of Cordyceps cardinalis were successfully produced in cereals. Brown rice, German millet and standard millet produced the longest-length of stromata, followed by Chinese pearl barley, Indian millet, black rice and standard barley. Oatmeal produced the shortest-length of fruiting bodies. Supplementation of pupa and larva to the grains resulted in a slightly enhanced production of fruiting bodies; pupa showing better production than larva. 50~60 g of brown rice and 10~20 g of pupa mixed with 50~60 mL of water in 1,000 mL polypropylene (PP) bottle was found to be optimum for fruiting body production. Liquid inoculation of 15~20 mL per PP bottle produced best fruiting bodies. The optimal temperature for the formation of fruiting bodies was 25degrees C, under conditions of continuous light. Few fruiting bodies were produced under the condition of complete darkness, and the fresh weight was considerable low, compared to that of light condition.
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