Mycobiology.
2010 Jun;38(2):108-112.
Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis
- Affiliations
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- 1BioRefinery Center, Korea Research Institute of Chemical Technology, Daejeon 305-600, Korea.
- 2Mass Spectrometry Research Center/Mass Spectrometry Team, Korea Basic Science Institute, Daejeon 305-333, Korea.
- 3Department of Microbiology and Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 305-764, Korea.
- 4Department of Microbiology and Biotechnology, Daejeon University, Daejeon 300-716, Korea. shinks@dju.kr
Abstract
- To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis. Differentially expressed proteins were identified by tandem mass spectrometry (MS/MS) and were compared with a protein database. In whole-cell extracts, 10 proteins were up-regulated and 9 proteins were down-regulated in the mutant. In extracellular preparations, 6 proteins were up-regulated in the lkh1 + null mutant and 4 proteins successfully identified: glycolipid anchored surface precursor, beta-glucosidase (Psu1), cell surface protein, glucan 1,3-beta-glucosidase (Bgl2), and exo-1,3 beta-glucanase (Exg1). These results suggest that Lkh1 is involved in regulating cell wall assembly.
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