Mycobiology.  2018 Sep;46(3):236-241. 10.1080/12298093.2018.1513115.

LAMMER Kinase Lkh1 Is an Upstream Regulator of Prk1-Mediated Non-Sexual Flocculation in Fission Yeast

• Park YD
• Kwon SJ
• Bae KS
• Park HM

• KMID: 2427104
• DOI: http://doi.org/10.1080/12298093.2018.1513115

Abstract

The cation-dependent galactose-specific flocculation activity of the Schizosaccharomyces pombe null mutant of lkh1⁺, the gene encoding LAMMER kinase homolog, has previously been reported by our group. Here, we show that disruption of prk1⁺, another flocculation associated regulatory kinase encoding gene, also resulted in cation-dependent galactose-specific flocculation. Deletion of prk1 increased the flocculation phenotype of the lkh1⁺ null mutant and its overexpression reversed the flocculation of cells caused by lkh1 deletion. Transcript levels of prk1⁺ were also decreased by lkh1⁺ deletion. Cumulatively, these results indicate that Lkh1 is one of the negative regulators acting upstream of Prk1, regulating non-sexual flocculation in fission yeast.

Keyword

Non-sexual flocculation; LAMMER kinase; Lkh1; Prk1; negative regulator; Schizosaccharomyces pombe

MeSH Terms

Flocculation*
Phenotype
Phosphotransferases*
Schizosaccharomyces*
Phosphotransferases