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LAMMER Kinase Lkh1 Is an Upstream Regulator of Prk1-Mediated Non-Sexual Flocculation in Fission Yeast

Park YD, Kwon SJ, Bae KS, Park HM

The cation-dependent galactose-specific flocculation activity of the Schizosaccharomyces pombe null mutant of lkh1⁺, the gene encoding LAMMER kinase homolog, has previously been reported by our group. Here, we show that...
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Screening Molecular Chaperones Similar to Small Heat Shock Proteins in Schizosaccharomyces pombe

Han J, Kim K, Lee S

To screen molecular chaperones similar to small heat shock proteins (sHsps), but without alpha-crystalline domain, heat-stable proteins from Schizosaccharomyces pombe were analyzed by 2-dimensional electrophoresis and matrix assisted laser desorption/ionization...
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Possible Roles of LAMMER Kinase Lkh1 in Fission Yeast by Comparative Proteome Analysis

Cho SJ, Kim YH, Park HM, Shin KS

  • KMID: 2312575
  • Mycobiology.
  • 2010 Jun;38(2):108-112.
To investigate the possible roles of LAMMER kinase homologue, Lkh1, in Schizosaccharomyces pombe, whole proteins were extracted from wild type and lkh1-deletion mutant cells and subjected to polyacrylamide gel electrophoresis....
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Heterologous Expression of Rhizopus Oryzae CYP509C12 Gene in Rhizopus Nigricans Enhances Reactive Oxygen Species Production and 11α-Hydroxylation Rate of 16α, 17-Epoxyprogesterone

Shen C, Gao X, Li T, Zhang J, Gao Y, Qiu L, Zhang G

The 11α-hydroxylation of 16α, 17-epoxyprogesterone (EP) catalyzed by Rhizopus nigricans is crucial for the steroid industry. However, lower conversion rate of the biohydroxylation restricts its potential industrial application. The 11α-steroid...
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Optimization of a microarray for fission yeast

Kim DU, Lee M, Han S, Nam M, Lee S, Lee J, Woo J, Kim D, Hoe KL

Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification of genes required for growth in any condition of interest. Anti-sense strands of amplified bar-codes hybridize with ~10,000 (5,000...
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Mutation Analysis of Synthetic DNA Barcodes in a Fission Yeast Gene Deletion Library by Sanger Sequencing

Lee M, Choi SJ, Han S, Nam M, Kim D, Kim DU, Hoe KL

Incorporation of unique barcodes into fission yeast gene deletion collections has enabled the identification of gene functions by growth fitness analysis. For fine tuning, it is important to examine barcode...
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