Lab Med Online.  2014 Jan;4(1):43-50.

Performance Comparison of ImmunoCAP and HYTEC 288 in the Quantitative Tests of Allergen-specific IgE

• Lee SH
• Lee SM
• Kim HH
• Chang CL
• Lee EY

Abstract

BACKGROUND
In vitro measurement of allergen-specific IgE has become an important part of allergy diagnoses. HYTEC 288 system (Hycor Biomedical Inc., USA), which was recently introduced in Korea, is a fully automated immunoassay for quantitative measurements of allergen-specific IgE. In this study, we compared the clinical utility of this in vitro allergy test with that of ImmunoCAP assay (ImmunoDiagnostics, Sweden).
METHODS
To evaluate the reproducibility of HYTEC 288 system, 50 serum samples were tested in duplicate each for Dermatophagoides pteronyssinus (d1) and D. farinae (d2) specific IgE. To assess the agreement between ImmunoCAP and HYTEC 288 assays, 56 serum samples were tested for the other 21 allergen-specific IgE.
RESULTS
No significant differences within the range of quantitative analysis were observed between HYTEC 288 and ImmunoCAP assays for d1 and d2 (P=0.65 and 0.55, respectively). The agreements of HYTEC allergen-specific IgE assay with ImmunoCAP within +/-1 class grade were 80% and 100% for d1 and d2, respectively. The correlation coefficients between HYTEC 288 and ImmunoCAP results within the range of quantitative analysis were overally 0.90, regardless of allergen, for d1 and d2 specific IgE, 0.91 and 0.98, respectively. Running times for the HYTEC 288 and Phardia 100 were 5.5 and 4.6 min per test, respectively.
CONCLUSIONS
Hycor HYTEC 288 showed a favorable agreement with ImmunoCAP and can be used for fully automated quantitative measurements of allergen-specific IgE in the clinical laboratory.

Keyword

Immunoglobulin E; Immunoassay; Allergy; Automation; Laboratory

MeSH Terms

Automation
Dermatophagoides pteronyssinus
Diagnosis
Hypersensitivity
Immunoassay
Immunoglobulin E*
Korea
Running
Immunoglobulin E

Figure

• 

  Fig. 1 Comparison of serum specific IgE levels measured by ImmunoCAP and HYTEC assays. (A, B) Scatter plot with Deming fit and residual plot of 84 allergen-specific IgE results within the range of quantitative analysis. (C, D) Scatter plot with Deming fit and difference plot of d1-specific IgE. (E, F) Scatter plot with Deming fit and difference plot of d2-specific IgE.

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