Clin Exp Otorhinolaryngol.  2015 Dec;8(4):385-389. 10.3342/ceo.2015.8.4.385.

Comparison of Component-Resolved Diagnosis by Using Allergen Microarray With the Conventional Tests in Allergic Rhinitis Patients: The First Using in Korea

Affiliations
  • 1Department of Otolaryngology-Head & Neck Surgery, Gachon University Gil Medical Center, Incheon, Korea. rhinokim2002@hanmail.net

Abstract


OBJECTIVES
The aim of this study was to evaluate the component-resolved diagnosis using a microarray allergen chip (Immuno Solid-phase Allergen Chip, ImmunoCAP ISAC) and to compare this new diagnostic tool with the established ImmunoCAP methods for allergen-specific IgE detection in allergic rhinitis patients.
METHODS
One hundred sixty-eight allergic rhinitis patients were included in this study. All the patients were diagnosed with allergic rhinitis according to their clinical symptoms, physical examination and a positive skin prick test. We analyzed their specific IgEs for house dust mites (Dermatophagoides farine [DF] and Dermatophagoides pteronyssinus [DP]), Alternaria alternata, birch, and mugwort using ImmunoCAP and ImmunoCAP ISAC in the same patient sample. We compared the sensitivity and correlation between the two tests.
RESULTS
In cases of allergies to DP and DF, the sensitivity of the specific IgE was 80% and that of the allergen microarray was 78.9%. The correlation between the two tests was significant for both DP and DF (P<0.001). For the A. alternata, birch and mugwort allergens, the sensitivity of ImmunoCAP ISAC was slightly lower than that of ImmunoCAP.
CONCLUSION
These results suggest that the allergen microarray chip method is a reliable new method to diagnose the components of an allergen in patients with allergic rhinitis sensitive to house dust mites. Further study about the utility of the allergen microarray is needed.

Keyword

Allergic Rhinitis; Specific IgE; Microarray

MeSH Terms

Allergens
Alternaria
Artemisia
Betula
Dermatophagoides pteronyssinus
Diagnosis*
Humans
Hypersensitivity
Immunoglobulin E
Korea*
Physical Examination
Pyroglyphidae
Rhinitis*
Skin
Allergens
Immunoglobulin E

Figure

  • Fig. 1 The comparison of sensitivity. (A) The specific IgE and allergen-microarray to Dermatophagoides farine and Dermatophagoides pteronyssinus. The sensitivity of the specific IgE was 80%, and that of the allergen-microarray was 78.9%. The Kappa value was 0.774. (B) The specific IgE and allergen microarray to fungus. The sensitivity of the specific IgE was 91.3%, and that of the allergen microarray was 76.9%. The Kappa value was 0.551. (C) The specific IgE and allergen microarray to tree pollen. The sensitivity of the specific IgE was 86.9%, and that of the allergen microarray was 43.6%. The Kappa value was 0.511. (D) The specific IgE and allergen microarray to weed pollen. The sensitivity of the specific IgE was 92.3%, and that of the allergen-microarray was 69.2%. The Kappa value was 0.670.

  • Fig. 2 The correlation of specific IgE vs. ImmunoCAP ISAC. (A) The specific IgE to Dermatophagoides pteronyssinus (DP) and nDer p 1. The correlation coefficient for nDer p 1 was 0.61, and the P-value was <0.001. (B) The specific IgE to DP and nDer p 2. The correlation coefficient for nDer p 2 was 0.70, and the P-value was <0.001. (C) The specific IgE to Dermatophagoides farine (DF) and nDer f 1. The correlation coefficient for nDer f 1 was 0.59, and the P-value was <0.001. (D) The specific IgE to DF and nDer f 2. The correlation coefficient for nDer f 2 was 0.55, and the P-value was <0.001. ISU-E, ISAC standardized unit.


Cited by  1 articles

Patients’ Characteristics according to Allergic Sensitization in Chronic Rhinitis
Chung Man Sung, Hyung Chae Yang, Hyong-Ho Cho
Korean J Otorhinolaryngol-Head Neck Surg. 2018;61(2):85-90.    doi: 10.3342/kjorl-hns.2017.00388.


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