Lab Anim Res.  2015 Sep;31(3):148-152. 10.5625/lar.2015.31.3.148.

Extraction conditions of white rose petals for the inhibition of enzymes related to skin aging

• Choi EK
• Guo H
• Choi JK
• Jang SK
• Shin K
• Cha YS
• Choi Y
• Seo DW
• Lee YB
• Joo SS
• Kim YB

• KMID: 2312132
• DOI: http://doi.org/10.5625/lar.2015.31.3.148

Abstract

In order to assess inhibitory potentials of white rose petal extracts (WRPE) on the activities of enzymes related to dermal aging according to the extraction conditions, three extraction methods were adopted. WRPE was prepared by extracting dried white rose (Rosa hybrida) petals with 50% ethanol (WRPE-EtOH), Pectinex(R) SMASH XXL enzyme (WRPE-enzyme) or high temperature-high pressure (WRPE-HTHP). In the inhibition of matrix metalloproteinase-1, although the enzyme activity was fully inhibited by all 3 extracts at 100 microg/mL in 60 min, partial inhibition (50-70%) was achieved only by WRPE-EtOH and WRPE-enzyme at 50 microg/mL. High concentrations (> or =250 microg/mL) of all 3 extracts markedly inhibited the elastase activity. However, at low concentrations (15.6-125 microg/mL), only WRPE-EtOH inhibited the enzyme activity. Notably, WRPE-EtOH was superior to WRPE-enzyme and WRPE-HTHP in the inhibition of tyrosinase. WRPE-EtOH significantly inhibited the enzyme activity from 31.2 microM, reaching 80% inhibition at 125 microM. In addition to its strong antioxidative activity, the ethanol extract of white rose petals was confirmed to be effective in inhibiting skin aging-related enzymes. Therefore, it is suggested that WRPE-EtOH could be a good candidate for the improvement of skin aging such as wrinkle formation and pigmentation.

Keyword

Rosa hybrida; white rose petal extract; matrix metalloproteinase-1; elastase; tyrosinase

MeSH Terms

Aging
Ethanol
Matrix Metalloproteinase 1
Monophenol Monooxygenase
Pancreatic Elastase
Pigmentation
Skin Aging*
Skin*
Ethanol
Matrix Metalloproteinase 1
Monophenol Monooxygenase
Pancreatic Elastase

Figure

• 

  Figure 1 Inhibition of matrix metalloproteinase-1 (MMP-1) by white rose petal extracts (WRPE). White rose petals were extracted with ethanol (WRPE-EtOH, A), enzyme (WRP-enzyme, B) or high temperature-high pressure (WRPE-HTHP, C). ■, blank (buffer alone); ▲, vehicle (MMP-1 alone); ▼, trypsin inhibitor; ○, 25 µg/mL WRPE; □, 50 µg/mL WRPE; ◇, 100 µg/mL WRPE.

• 

  Figure 2 Inhibition of elastase by white rose petal extracts (WRPE). White rose petals were extracted with ethanol (WRPE-EtOH, A), enzyme (WRP-enzyme, B) or high temperature-high pressure (WRPE-HTHP, C). *, **, ***Significantly different from vehicle control (Ctrl) (*<0.05, **<0.01, ***<0.001).

• 

  Figure 3 Inhibition of tyrosinase by white rose petal extracts (WRPE). White rose petals were extracted with ethanol (WRPE-EtOH, A), enzyme (WRP-enzyme, B) or high temperature-high pressure (WRPE-HTHP, C). *, **, ***Significantly different from vehicle control (Ctrl) (*<0.05, **<0.01, ***<0.001).

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