Lab Anim Res.
2010 Jun;26(2):139-144. 10.5625/lar.2010.26.2.139.
Fractionation of DNases Specific to Haemonchus contortus Intestine by Phenyl Sepharose Column
- Affiliations
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- 1College of Veterinary Medicine, Kyungpook National University, Daegu, Korea. dmkwak@knu.ac.kr
- KMID: 2312059
- DOI: http://doi.org/10.5625/lar.2010.26.2.139
Abstract
- Multiple DNases were identified from Haemonchus contortus intestine based on previous studies. The DNases detected at 34, 36 and 38.5 kDa had diverse characteristics. Some of them had characteristics similar to those of mammalians and others had unusual characteristics. This study was carried out to fractionate worm intestinal DNases from other proteins using phenyl Sepharose chromatographic methods. All DNases detected from Haemonchus contortus intestine were fractionated in the flowthrough of phenyl Sepharose, indicating the worm DNases are hydrophilic. The DNases were enriched five-fold in the flowthrough fraction while additional steps are required for isolation of the worm DNases. Thus, fractionation with phenyl Sepharose could be used as a good initial step to enrich and separate DNases from other proteins.
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