Experience with 671 peripheral blood stem cell collection

Whang, Dong Hee; Hur, Mina; Park, Kyoung Un; Shin, Soo; Kim, Yang Hyun; Shin, Hee Young; Ahn, Hyo Seop; Park, Seonyang

Abstract

BACKGROUND
S: Peripheral blood stem cells (PBSC) are increasingly used because of ease of collection and rapid kinetics of recovery relative to bone marrow transplantation (BMT). Here we summarize laboratory and clinical experiences of 671 PBSC harvests performed at a single institution.
METHODS
PBSC harvests were performed mainly with Fenwal CS3000 plus blood cell separator. 126 cancer patients and 12 allogeneic BMT donors (54 children, 84 adults) were enrolled in this study. Total WBC and mononuclear cells (MNC) were analyzed in peripheral blood before procedure, and total WBC, MNC and CD34+ cell count on PBSC product. Correlation between dose of CD34+ cells and hematologic recovery was evaluated by Wilcoxon rank sum test and Pearson correlation analysis. RESLUTS: A mean of 4.8 leukapheresis was performed and 9,155 mL blood was processed. A mean volume of PBSC product was 66 mL and MNC purity of that was 84%. An average of 3.0 +/- 3.3x108 WBC/kg, 2.4 +/- 2.6x108 MNC/kg, and 2.7 +/- 5.4x106 CD34+ cells/kg was collected in each procedure. A goal of 1x108/kg MNCs was reached in 98.4% (127/129) of the patients and more than 1x106/kg CD34+ cells were collected in 91.8% (90/98). Various complications of PBSC harvests were observed but the incidence of serious adverse reaction was low. Median times to an absolute neutrophil count over 500/uL were 11 (7-34) and 11 (8-29) days respectively, in adult and children, and those to a sustained platelet count over 50,000/uL were 18.5 (9-118) and 36 (16-85) days, respectively.
CONCLUSION
We analyzed yield, adverse reaction and hematologic recovery of PBSC harvests and this can serve basic data for the PBSC procedure.