Cutoff Value of PB CD34+ Cells for Optimization of PBSC Collection

Lee, Hyun Ji; Shin, Kyung Hwa; Song, Dual; Kim, Shine Young; Kim, Hyung Hoi; Lee, Eun Yup; Son, Han Chul

Abstract

BACKGROUND
Peripheral hematopoietic stem cell mobilization is increasing due to its advantages. For successful engraftment, obtaining sufficient stem cells is prerequisite. The number of CD34+ cells of collected blood are widely used to predict the engraftment potential. To determine the optimal point for collection of peripheral blood stem cell (PBSC), enumeration of the number of CD34+ cells in peripheral blood (PB) is known to be helpful. The purpose of this study is to analyze cutoff value of CD34+ cells in PB.
METHODS
We analyzed 407 cases of autologous PBSC collection and 107 cases of allogenic PBSC collection during 2004~2009 in Pusan National University Hospital. Complete blood count, HPC fraction and number, CD34+ cells in PB and product of PBSC collection were analyzed.
RESULTS
The each number of mononuclear cells and HPC in PB showed a strong correlation with CD34+ cells in PB. A strong correlation between the number of circulation CD34+ cells in PB on the day of collection and the number of collected CD34+ cells was found. The ROC curve revealed that the cutoff point having the optimal sensitivity and specificity at 8.5/uL for target CD34+ cells > or =1.0x10(6)/kg, 10.5/uL for target CD34+ cells > or =1.5x10(6)/kg and 13.5/uL for target CD34+ cells > or =2.0x10(6)/kg in this study.
CONCLUSION
To obtain a sufficient yield of CD34+ cells during PBSC collection, determination of cut off point for each target CD34+ cells//kg is helpful to decide the collection.