Korean J Blood Transfus.  2010 Dec;21(3):280-288.

Isolation of Mesenchymal Stem Cells from the Mononuclear Cells Remaining in the Bone Marrow Processing Kit

Affiliations
  • 1Department of Laboratory Medicine, Yonsei University College of Medicine, Seoul, Korea. hyunok1019@yuhs.ac
  • 2Yonsei Cell Therapy Center, Yonsei University College of Medicine, Seoul, Korea.

Abstract

BACKGROUND
Mesenchymal stem cells (MSCs) that are capable of extensive self renewal and differentiation have attracted great attention as a promising tool for regenerative medicine and tissue engineering. Although MSCs can be isolated from various tissues, bone marrow currently represents one of the most reliable sources for providing a sufficient yield of cells in a good quality. Herein, used bone marrow processing kits were evaluated as a valuable source of MSCs.
METHODS
Bone marrow mononuclear cells (MNCs) were recovered from used bone marrow processing kits after routine bone marrow processing by using the COBE 2991 Cell Processor (CaridianBCT Inc.). The MSCs were isolated from the recovered MNCs using a standard plastic adherence method. Immunophenotyping and differentiation assays were performed to clarify the characteristics of the isolated MSCs.
RESULTS
An average of 1x108 bone marrow MNCs was collected, and the MSCs were successfully isolated from the recovered bone marrow MNCs in all case. The isolated MSCs were positive for essential MSC surface molecules (CD29, CD44, CD73, CD90, CD105) and they were negative for most hematopoietic and endothelial cell markers (CD34, CD45, CD31, CD14). The isolated MSCs were capable of differentiation along the osteogenic, adipogenic and chondrogenic pathways.
CONCLUSION
MSCs isolated from used bone marrow processing kits are an alternative and ethical source of bone marrow derived MSCs, and they can be used for research purposes.

Keyword

Mesenchymal stem cell; Mononuclear cell; Bone marrow processing kit

MeSH Terms

Bone and Bones
Bone Marrow
Durapatite
Endothelial Cells
Immunophenotyping
Mesenchymal Stromal Cells
Plastics
Regenerative Medicine
Tissue Engineering
Durapatite
Plastics
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