Korean J Androl.
2010 Dec;28(3):163-168.
Monitoring Transplanted Human Mesenchymal Stem Cells in the Penile Cavernosal Tissues of Streptozotocin-induced Diabetic Rats Using Molecular Magnetic Resonance Imaging
- Affiliations
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- 1Department of Urology, Soonchunhyang University College of Medicine, Seoul, Korea. yssong@hosp.sch.ac.kr
- 2Department of Radiology, Soonchunhyang University College of Medicine, Seoul, Korea.
- 3Department of Surgery, Soonchunhyang University College of Medicine, Seoul, Korea.
- 4Department of Internal Medicine, Soonchunhyang University College of Medicine, Seoul, Korea.
Abstract
- PURPOSE
A molecular magnetic resonance (MR) imaging technique using superparamagnetic iron oxide (SPIO) nanocrystals was developed for monitoring stem cells noninvasively. This study was performed to investigate whether the presence of transplanted human mesenchymal stem cells in the penile cavernosum of a diabetic rat model could be evaluated noninvasively using molecular MR imaging.
MATERIALS AND METHODS
SPIO nanocrystals (Feridex; AMI, Cambridge, USA) were transferred to human mesenchymal stem cells (hMSCs) using GenePORTER. The SPIO-transferred hMSCs were examined with Prussian blue staining. SPIO-labeled hMSCs were transplanted to the penile cavernosum of a diabetic rat model and MR images were examined in vivo using 1.5 T MR.
RESULTS
SPIO was transferred to hMSCs successfully. MR signal intensity at the areas of the SPIO-transferred hMSCs decreased in the penile cavernosum of the diabetic rat model. SPIO particles were confirmed in the transplanted penile cavernosum with Prussian blue staining.
CONCLUSIONS
The SPIO-labelled hMSCs in the penile cavernosum of a diabetic rat model can be monitored noninvasively using molecular MR imaging.
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