Korean J Aerosp Environ Med.  2005 Apr;15(1):18-22.

Effect of Antioxidants on Aminoglycoside-induced Cochlear Cell Damage

• Nam SI

Abstract

BACKGROUND
Aminoglycosides that are widely used in the treatment of Gram (-)-infected diseases have side effects such as ototoxicity and nephrotoxicity. These side effects are closely related to oxidative stress. The purpose of this study was to evaluate the effect of antioxidants on the aminoglycoside-induced cochlear cell damage.
METHODS
HEI-OC1 was used as an experimental cell line. The number of cells was counted after administration of streptomycin or gentamicin plus Vitamin E, taurine, or one of the components of green tea (EGCG, EGC, ECG, EC) under cell proliferating conditions (33oC).
RESULTS
Streptomycin and gentamicin decreased the number of HEI-OC1 cells in a dose dependent manner. Aminoglycoside-induced cell death was recovered by the addition of Vitamin E, taurine, or green tea.
CONCLUSION
These results suggest that Vitamin E, taurine, or green tea components block aminoglycoside-induced ototoxicity via the suppression of the increased production of reactive oxygen species.

Keyword

Ototoxicity; Aminoglycoside; Antioxidant

MeSH Terms

Aminoglycosides
Antioxidants*
Cell Death
Cell Line
Electrocardiography
Gentamicins
Oxidative Stress
Reactive Oxygen Species
Streptomycin
Taurine
Tea
Vitamin E
Vitamins
Aminoglycosides
Antioxidants
Gentamicins
Reactive Oxygen Species
Streptomycin
Taurine
Tea
Vitamin E
Vitamins