Korean J Urol.  2003 Feb;44(2):155-159.

Changes of Nitric Oxide and Cell Proliferation after Bacillus Calmette-Guerin and Nitric Oxide Synthase Inhibitor Treatment on Murine Bladder Tumor-2 Cell Line

• Ryu SB
• Oh BR
• Kang TW
• Oh SH
• Nah HS
• Lee HC

Abstract

PURPOSE: We hypothesize that the cytolytic activity of Bacillus Calmette-Guerin (BCG) may act through nitric oxide (NO) production, and that cell death is correlated with apoptosis, changes of NO and cell proliferation following BCG and/or nitric oxide synthase (NOS) inhibition treatment of a murine bladder tumor-2 (MBT-2) cell line. If these cell lines show cell death due to apoptosis was also determined.
MATERIALS AND METHODS
NO production and proliferation activity of the MBT-2 cell line were measured after stimulation, with BCG and/or L-NAME, using an enzyme- linked immunosorbent assay (ELISA). After incubation of the MBT-2 cells with BCG and/or L-NAME, the cell cycle was analysis was performed by immunocytometry.
RESULTS
The production of NO in the MBT-2 cells was significantly increased by the BCG. The BCG had direct dose-dependent cytotoxic effects on the MBT-2 cell line. After the L-NAME treatment, both the NO production and cytotoxicity were decreased (p<0.05). When the MBT-2 cells were cultured with BCG, the apoptotic cell ratio increased compared to that of the MBT-2 cells treated with L-NAME (p<0.05).
CONCLUSIONS
The up-regulation of the production of NO following BCG treatment of the MBT-2 cell line may be due, in part, to the cytolytic action of the BCG. The cell death, when BCG was instilled, correlated with apoptosis.

Keyword

Bacillus Calmette-Guerin; MBT-2 cell line; Nitric oxide; Nitric oxide synthase inhibitor; Apoptosis

MeSH Terms

Apoptosis
Bacillus*
Cell Cycle
Cell Death
Cell Line*
Cell Proliferation*
Mycobacterium bovis
NG-Nitroarginine Methyl Ester
Nitric Oxide Synthase*
Nitric Oxide*
Up-Regulation
Urinary Bladder*
NG-Nitroarginine Methyl Ester
Nitric Oxide
Nitric Oxide Synthase