Korean J Thorac Cardiovasc Surg.
1998 Dec;31(12):1134-1146.
Effect of Adenovirus-p53 to Non-Small Cell Lung Cancer Cell Lines
- Affiliations
-
- 1Dept. of Thoracic Surgery, Korea Cancer Center Hospital, Seoul, Korea.
- 2Dept. of Internal Medicine, Korea Cancer Center Hospital, Seoul, Korea.
- 3Dept. of Thoracic & Cardiovascular Surgery, Seoul National University Hospital, Seoul, Korea.
Abstract
-
BACKGROUND: The tumor suppressor gene p53 is one of the most frequently altered genes in human tumors, including those of the lung. There is now a compelling evidence that wild-type p53 can negatively influence cell growth by causing G1 arrest or by inducing apoptosis. The possibilities of using p53 for gene therapy are also gathering much interest.
MATERIAL AND METHOD: Our approach towards understanding p53 function would be to study the biological consequences of overexpression of wild-type p53 in normal and tumor cells by using adenovirus vectors capable of giving high levels of the p53 gene product in cells. We have used this vector containing wild-type p53 to infect tumor cells with different p53 status (null, mutant, or wild-type) to confirm that expression of p53 in null or mutant cell lines becomes possible by Adenovirus-p53 transduction, to examine the effects of high levels of p53 expression on the growth properties of tumor cells, to evaluate the role of apoptosis in p53-mediated biological effects, and to examine the effect of Adenovirus-p53 on the tumorigenicities of the lung cancer cell lines in vitro.
RESULT: The results of our study showed that cells expressing endogenous mutant p53 and those devoid of p53 expression altogether were significantly more sensitive to Adenovirus-p53-mediated cytotoxicity compared to tumor cells expressing endogenous wild-type p53 and that overexpression of wild-type p53 induced programmed cell death. Also we knew that Adenovirus-p53 significantly reduced tumor colony formation of human non-small cell lung cancer cell lines, and decreased the growth of preformed colonies in vitro.
CONCLUSION
These results suggest that adenovirus is an efficient vector for mediating transfer and expression of tumor suppressor genes in human non-small cell lung cancer cells and that the tumor cells null for p53 or expressing mutant p53 readily undergo apoptosis by Adenovirus-p53.
Keyword
- Full Text Links
-
- Actions
-
Cited
- CITED
-
- Close
- Share
-
- Similar articles
-
- Limited Cytotoxic Effect of Adenoviral-mediated p53 Gene Transfer in Variable Non-small Cell Lung Cancer (NSCLC) Cell Lines
- The Synergistic Cell Killing Effects by the Transduction of the w-p53 Gene and 5-FU Administration in Colon Cancer Cell Lines
- Enhanced Growth inhibition by Combined Gene Transfer of p53 and p16INK4a in Adenoviral Vectors to Lung Cancer Cell Lines
- Expression of p53, bcl-2 Protein in Small Cell Lung Cancer (SCLC) Cell Lines in Relation to Sensitivity to Chemotherapy
- Adenovirus - Mediated gene Transfer of Wild - Type p53 Results in Restoration of Tumor - Suppressor Function in Glioma Cell Lines
