J Clin Pathol Qual Control.
1999 Dec;21(2):379-387.
Experience of International Cell Exchange Program for HLA DNA Typing
- Affiliations
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- 1Department of Clinical Pathology, Hallym University College of Medicine, Seoul, Korea.
Abstract
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BACKGROUND: Due to high accuracy and reproducibility, and consistency of the typing assignments, the use of HLA DNA typing has been increasing. Kangdong Sacred Heart Hospital has participated in International Cell Exchange Program (ICEP) which to one of the most well known external HLA DNA proficiency testing program, In this report, data obtained from ICEP samples were analyzed to assess advantages and disadvantages from the participation.
METHODS
Accuracy and error rates were calculated by comparing reported types obtained from 24 samples (1999. 2 - 1999. 7) for class I and from 86 samples (1995. 2 - 1999. 6) for class II to original types from ICEP monthly reports. For class II, data from 56 (PCR-SSOP L 1995-1997) and 30 (PCR-SBT : 1998-1999) samples were analyzed separately based on the methods used in typing. In addition, several trials were made for samples exhibiting errors in assignment to improve accuracy.
RESULTS
Accuracies for HLA-A, -B, and -C were 95.6%, 95.8%, and 94.5% respectively. For HLA class II typing data obtained by PCR-SSOP method, accuracies for HLA-DRB1 and -DQB1 were 91.0% and 92.7% respectively. False positivity and negativity of SSOPs used in the typing appeared to be the major source of errors. For data obtained from 30 samples typed by PCR-SBT, accuracy for HLA-DRB1 and -DQB1 were 100% and 94.1% respectively. To improve accuracy of the HLA typing, newly designed primers for PCR and DNA sequencing reaction as well as duplicate analysis of DNA sequencing histogram by two experts were applied to inaccurately assigned samples.
CONCLUSIONS
The participation in ICEP was effective to improve reliability and quality control of HLA DNA typing system established in our laboratory, in addition to acquisition of various updated informations on HLA typing. However, it is necessary to organize more economic and efficient proficiency testing program in Korea in considering ethnical background of supported samples and costs spent in ICEP.
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