J Clin Pathol Qual Control.  1999 Dec;21(2):329-334.

Comparison of the Reagents in Automated Hematology Analyzer Coulter STKS and Cell-Dyn 1300

• Lee CH
• Park YJ
• Park JS
• Lee KA
• yoon KJ

Abstract

BACKGROUND: Routine CBC analysis is one of the most fundamental and informative test in the clinical laboratory, Recently, some opened reagents for automated hematology analyzer have been introduced in our country. So, we evaluated the efficiency of the opened reagents in the Coulter STKS (Beckmann-Coulter Corp., Miami, FL, USA) and in the Cell-Dyn 1300 (Abbott laboratories, IL, USA) analyzer by comparing with the original reagents.
METHODS
From 210 patients' samples, correlation analysis for 16 items (WBC, RBC, Hb, Hct, MCV, MCH, MCHC, RDW, PLT, MPV, PDW, Neu, Lym, Mono, Eos, Baso) in the Coulter STKS and for 8 items (WBC, RBC, HB, Hct, MCV, MCH, MCHC, PLT) in the Cell-Dyn 1300 were performed. The opened reagents were J.T. Baker (Mallinckrodt Baker Inc., Holland) for the Coulter STKS and LABNOVA (Labnova s.r.l. Italy) for the Cell-Dyn 1400, respectively. Within-run, between-run, and between-day precision tests of the opened reagents were evaluated from 10 patients' samples.
RESULTS
Correlation analysis revealed good results (p<0.001, r>0.960) in most items of the two analyzers except MCHC (r=0.701), monocyte (r=0.924), and basophil (r=0.363) in the Coulter STKS, but these were thought to be clinically not significant. Coefficient of variation (CV) of within-run. between-run, and between-day precision were less than 1-5% in all items of the two analyzers except between-day precision of platelet count (CV=14.2%) in the Coulter STKS.
CONCLUSION
We concluded that these two opened reagents for the Coulter STKS and the Cell-Dyn 1300 showed good correlation and precision in comparison with the original reagents. But further reevaluation and careful quality control may be required for platelet count in the Coulter STKS, and also further study for neonates and hematologic disorder patients including peripheral blastic cells.

MeSH Terms

Basophils
Hematology*
Humans
Indicators and Reagents*
Infant, Newborn
Monocytes
Platelet Count
Quality Control
Indicators and Reagents