Korean J Physiol Pharmacol.  2015 Sep;19(5):473-478. 10.4196/kjpp.2015.19.5.473.

The Inhibitory Mechanism of Gentamicin on Electrical Field Stimulation Response in Rat Bladder Smooth Muscle

• Min CH
• Wang Y
• Bae J
• Han JH
• Sohn UD

• KMID: 2285593
• DOI: http://doi.org/10.4196/kjpp.2015.19.5.473

Abstract

To see the inhibitory mechanism of gentamicin in response to electrical field stimulation (EFS) using the rat bladder smooth muscle, atropine or guanethidine was treated but had no effect. Methylsergide, a non-selective 5-HT1, 5-HT2 receptor antagonist was also treated but had on effect. Kinase inhibitors, such as chelerythrine (PKC inhibitor), ML-9 (MLCK inhibitor), or Y27632 (rho kinase inhibitor) were pretreated before gentamicin treatment, but did not have effect. For U73122, a phospholipase C (PLC) inhibitor however, the inhibitory effect to gentamicin was significantly attenuated in all frequencies given by the EFS. Therefore gentamicin induced inhibitory effect on EFS response in rat bladder smooth muscle was not mediated by the activation of adrenergic, cholinergic, or serotonergic receptor. The inhibition of gentamicin might be mediated through the PLC dependent pathway, but not through the PKC, MLCK or rho kinase dependent pathway.

Keyword

EFS; Gentamicin; Phospholipase C; Smooth muscle

MeSH Terms

Animals
Atropine
Gentamicins*
Guanethidine
Muscle, Smooth*
Phosphotransferases
Rats*
rho-Associated Kinases
Type C Phospholipases
Urinary Bladder*
Atropine
Gentamicins
Guanethidine
Phosphotransferases
Type C Phospholipases
rho-Associated Kinases

Figure

• 

  Fig. 1 Representative tracing showing the effect of gentamycin on EFS in the rat bladder smooth muscle. Gentamicin was added cumulatively with an interval of 5 min. The tone was measured under the EFS of 1, 2, 4, 6, 8 Hz. Each point represents the mean±SE (n=8).

• 

  Fig. 2 Effect of atropine, guanethidine and methylsergide on EFS in rat bladder smooth muscle treated with gentamicin. Atropine (0.1 mM), guanethidine (0.1 mM) or rmethylsergide (10 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).

• 

  Fig. 3 Effect of chelerythrine, ML-9 and Y27632 on EFS in rat bladder smooth muscle treated with gentamicin. Chelerythrine (10 µM), ML-9 (10 µM) and Y27632 (1 µM) was treated 30 min prior to EFS stimulation. The tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8).

• 

  Fig. 4 Effect of U73122 on EFS in rat bladder smooth muscle treated with gentamicin. U73122 (1 µM) was treated 30 min prior to EFS stimulation. For each concentration the tone was measured under the EFS of 1~8 Hz. Each point represents the mean±SE (n=8). *p<0.05, **p<0.01 vs. control.

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