Korean J Physiol Pharmacol.  2014 Aug;18(4):327-331. 10.4196/kjpp.2014.18.4.327.

Fucoidan Promotes the Reconstruction of Skin Equivalents

• Song YS
• Li H
• Balcos MC
• Yun HY
• Baek KJ
• Kwon NS
• Choi HR
• Park KC
• Kim DS

• KMID: 2285527
• DOI: http://doi.org/10.4196/kjpp.2014.18.4.327

Abstract

In this study we investigated the effects of fucoidan on the proliferation of fibroblasts and the reconstruction of a skin equivalent (SE). Fucoidan significantly stimulated the proliferation of CCD-25Sk human fibroblasts and Western blot analysis demonstrated that fucoidan markedly increased the expression of cyclin D1 and decreased the expression of p27. Fucoidan was used to reconstruct SE. Immunohistochemical staining showed that the addition of fucoidan to dermal equivalents increased expression of proliferating cell nuclear antigen (PCNA) and p63. In addition, expression of alpha6-integrin was significantly increased by fucoidan, whereas expression of beta1-integrin, type 1 collagen, elastin, fibronectin did not markedly change. These results suggest that fucoidan has positive effects on epidermal reconstruction and will therefore be beneficial in the reconstruction of SE.

Keyword

Fibroblasts; Fucoidan; Proliferation; Skin equivalent

MeSH Terms

Blotting, Western
Collagen Type I
Cyclin D1
Elastin
Fibroblasts
Fibronectins
Humans
Proliferating Cell Nuclear Antigen
Skin*
Collagen Type I
Cyclin D1
Elastin
Fibronectins
Proliferating Cell Nuclear Antigen

Figure

• 

  Fig. 1 Effects of fucoidan on CCD-25Sk cell proliferation. Cells were treated with 0~50 µg/ml fucoidan for 3 days. (A) Cell proliferation was measured using the MTT assay. Data represent the means±S.D. of triplicate assays expressed as percentages of the control. *p<0.01 (B) Whole cell lysates were subjected to Western blot analysis with antibodies against phospho-specific ERK, cyclin D1, and p27. Equal protein loading was confirmed using an antiactin antibody. Fold increases over the control were determined by densitometric analysis and are shown below in each lane.

• 

  Fig. 2 Effects of fucoidan in the SE reconstruction. (A) Photographs of skin equivalents (SEs) in 24-mm transwell culture after 12 days of SE reconstruction. (B) SE sections were stained for H&E, PCNA, p63, α6-integrin, β1-integrin, type I collagen, elastin, and fibronectin. Each experiment was repeated independently three times, and representative results are shown. Original magnification, ×400. (C) PCNA, p63, and α6-integrin intensities were measured as described in 'Materials and Methods.' *p<0.01 compared to the control model.

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