Nutr Res Pract.  2023 Oct;17(5):844-854. 10.4162/nrp.2023.17.5.844.

Antimetastatic effect of fucoidan against non-small cell lung cancer by suppressing non-receptor tyrosine kinase and extracellular signalrelated kinase pathway

• Muneerungsee N
• Tanasawet S
• Sukketsiri W

• KMID: 2546943
• DOI: http://doi.org/10.4162/nrp.2023.17.5.844

Abstract

BACKGROUND/OBJECTIVES
Fucoidan, a polysaccharide content in brown algae, has been reported to inhibit the growth of cancer cells. The present study aimed to investigate the suppression effects of fucoidan on A549 non-small cell lung cancer cells migration.
MATERIALS/METHODS
The anti-migratory activity of fucoidan in A549 cells was examined by wound healing assay and phalloidin-rhodamine staining in response to fucoidan (0–100 µg/mL) treatment for 48 h. Western blot analysis was performed to clarify the protein expressions relevant to migratory activity.
RESULTS
Fucoidan (25–100 µg/mL) significantly suppressed A549 cells migration together with reduced the intensity of phalloidin-rhodamine which detect filopodia and lamellipodia protrusions at 48 h of treatment. The protein expression indicated that fucoidan significantly suppressed the phosphorylation of focal adhesion kinase (FAK), Src, and extracellular signalrelated kinase (ERK). In addition, the phosphorylation of p38 in A549 cells was found to be increased.
CONCLUSIONS
Our data conclude that fucoidan exhibits anti-migratory activities against lung cancer A549 cells mediated by inhibiting ERK1/2 and FAK-Src pathway.

Keyword

Fucoidan; non-small cell lung cancer; filopodia; metastasis