Korean J Physiol Pharmacol.
1998 Aug;2(4):419-425.
Effects of exogenous ATP on calcium mobilization and cell proliferation in C6 glioma cell
- Affiliations
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- 1Department of Pharmacology, Catholic University Medical College, 505 Banpo-dong, Seocho-gu, Seoul 137-701 South Korea.
Abstract
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To clarify the effect of extracellular ATP in cultured C6 glioma cells,
ATP-induced cytosolic free calcium ((Ca2+)i) mobilization and cell
proliferation were investigated. ATP-induced (Ca2+)i increased in a
dose-dependent manner (10-7 M apprx 10-3 M). ATP-induced (Ca2+)i
increases were slightly slowed in extracellular calcium-free conditions
especially in sustained phase. ATP-induced (Ca2+)i increment was also
inhibited by the pretreatment of U73122, a phospholipase C (PLC)
inhibitor, in a time-dependent manner. Suramin, a putative P2Y receptor
antagonist, dose-dependently weakened ATP-induced (Ca2+)i mobilization.
Significant increases in cell proliferation were observed at 2, 3, and
4 days after ATP was added. Stimulated cell proliferation was also
observed with adenosine at days 2 and 3. This cell proliferation was
significantly inhibited by the treatment with suramin. Ionomycin also
stimulated cell proliferation in a concentration-dependent manner. In
conclusion
, we suggest that extracellular ATP stimulates C6 glioma cell proliferation via intracellular free calcium mobilization mediated by purinoceptor.
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