J Adv Prosthodont.  2014 Oct;6(5):406-414. 10.4047/jap.2014.6.5.406.

Cellular viability and genetic expression of human gingival fibroblasts to zirconia with enamel matrix derivative (Emdogain(R))

Affiliations
  • 1Department of Oral and Maxillofacial Surgery, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea.
  • 2Department of Oral Anatomy, Dental School, Kangnung-Wonju National University, Kangnung, Republic of Korea.
  • 3Department of Prosthodontics and Operative Dentistry, Tufts University School of Dental Medicine, Boston, MA, USA.
  • 4Department of Prosthodontics, School of Dentistry, Kyung Hee University, Seoul, Republic of Korea. ahranp@khu.ac.kr

Abstract

PURPOSE
The objective of this study was to investigate the biologic effects of enamel matrix derivative (EMD) with different concentrations on cell viability and the genetic expression of human gingival fibroblasts (HGF) to zirconia surfaces.
MATERIALS AND METHODS
Immortalized human gingival fibroblasts (HGF) were cultured (1) without EMD, (2) with EMD 25 microg/mL, and (3) with EMD 100 microg/mL on zirconia discs. MTT assay was performed to evaluate the cell proliferation activity and SEM was carried out to examine the cellular morphology and attachment. The mRNA expression of collagen type I, osteopontin, fibronectin, and TGF-beta1 was evaluated with the real-time polymerase chain reaction (RT-PCR).
RESULTS
From MTT assay, HGF showed more proliferation in EMD 25 microg/mL group than control and EMD 100 microg/mL group (P<.05). HGFs showed more flattened cellular morphology on the experimental groups than on the control group after 4h culture and more cellular attachments were observed on EMD 25 microg/mL group and EMD 100 microg/mL group after 24h culture. After 48h of culture, cellular attachment was similar in all groups. The mRNA expression of type I collagen increased in a concentration dependent manner. The genetic expression of osteopontin, fibronectin, and TGF-beta1 was increased at EMD 100 microg/mL. However, the mRNA expression of proteins associated with cellular attachment was decreased at EMD 25 microg/mL.
CONCLUSION
Through this short term culture of HGF on zirconium discs, we conclude that EMD affects the proliferation, attachment, and cell morphology of HGF cells. Also, EMD stimulates production of extracellular matrix collagen, osteopontin, and TGF-beta1 in high concentration levels. CLINICAL RELEVANCE: With the use of EMD, protective barrier between attached gingiva and transmucosal zirconia abutment may be enhanced leading to final esthetic results with implants.

Keyword

Enamel matrix derivative (Emdogain(R)); Human gingival fibroblast; Zirconia; Cell proliferation; Cell attachment

MeSH Terms

Cell Proliferation
Cell Survival
Collagen
Collagen Type I
Dental Enamel*
Extracellular Matrix
Fibroblasts*
Fibronectins
Gingiva
Humans
Osteopontin
Real-Time Polymerase Chain Reaction
RNA, Messenger
Transforming Growth Factor beta1
Zirconium
Collagen
Collagen Type I
Fibronectins
Osteopontin
RNA, Messenger
Transforming Growth Factor beta1
Zirconium
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