Korean J Prev Med.  1995 Mar;28(1):141-152.

Effects of Ethanol on the Activities and Inducibility of Trichloroethylene Metabolic Enzyme System in Rat Liver

• Kim KW
• Kang SK
• Cho YS
• Lee SH
• Moon YH
• Choi BS
• Park SS

Abstract

This study was performed to find out the influences of ethanol on the metabolism of trichloroethylene(TRI) in rats. TRI in corn oil at the dosage of 150, 300, 600 mg/kg was injected peritoneally once a day for two days to two groups. In one group ethanol(4 g/kg) was taken orally 30 minutes before TRI injection, and the other group ethanol was not. The results of experiments are as follows: 1. The contents of cytochrome P-450 and b5 had inverse relationship with in-jected TRI amounts in both groups. 2. The activity of NADPH P-450 reductase was decreased slowly in TRI injected group related with TRI amount, but decreased drastically in the group pretreated with ethanol. 3. The activity of NADH b5 reductase had relationship with injected TRI amount, but the statistical significance was found only in the groups of 300 and 600 mg/kg of TRI injected without relevance to ethanol when compared with the group that was not injected. 4. The activity of ADH was more decreased and ALDH activity was more increased in groups that TRI injected and ethanol was pretreated with ethanol groups than in group without any treatment. These results suggest that ethanol may inhibit epoxide formulation, the first step or TRI metabolism, and change from TCE-OH to TCA also.

Keyword

Trichloroethylene; Ethanol; Cytocchrome P-450 Monooxygenase

MeSH Terms

Animals
Corn Oil
Cytochrome P-450 Enzyme System
Ethanol*
Liver*
Metabolism
NAD
NADP
Oxidoreductases
Rats*
Trichloroethylene*
Corn Oil
Cytochrome P-450 Enzyme System
Ethanol
NAD
NADP
Oxidoreductases
Trichloroethylene