Korean J Pediatr.  2010 Aug;53(8):795-800. 10.3345/kjp.2010.53.8.795.

Increased B cell-activating factor (BAFF) level in the sputum of children with asthma

• Jee HM
• Choi BS
• Kim KW
• Sohn MH
• Han MY
• Kim KE

• KMID: 2280934
• DOI: http://doi.org/10.3345/kjp.2010.53.8.795

Abstract

PURPOSE
B cell-activating factor (BAFF) is a tumor-necrosis factor (TNF) superfamily member best known for its role in the survival and maturation of B cells. BAFF activity is observed in naive cells as well as in effector/memory T cells. We aimed to explore whether BAFF in sputum is expressed at elevated levels in asthmatic airways and associated with eosinophilic inflammation, pulmonary function, and bronchial hyperresponsiveness in children.
METHODS
One hundred and fifty-four asthmatic children and 98 healthy children were enrolled in the study. Sputum supernatants were collected and sputum BAFF and eosinophil cationic protein (ECP) levels were measured. We performed pulmonary function tests and methacholine challenge tests, while measuring total eosinophil count, total serum IgE, and serum ECP in all subjects.
RESULTS
Asthmatic children had significantly higher levels of BAFF in induced sputum [26.50 (10.50-100.27) pg/mL] compared to healthy children [18.32 (7.68-44.63) pg/mL; P=0.011]. Sputum BAFF positively correlated with sputum eosinophils (gamma=0.406, P<0.001) and sputum ECP (gamma=0.789, P<0.001). Significant negative correlations were found between sputum BAFF and FEV1 (gamma=-0.291, P<0.001) or post-bronchodilator FEV1 (gamma=-0.334, P<0.001), whereas nonsignificant correlations were found between sputum BAFF and bronchial hyperresponsiveness, serum eosinophil count, and serum ECP.
CONCLUSION
These findings suggest that BAFF may play a role in childhood asthma, and BAFF levels in sputum could be a supportive marker that represents airway inflammation, especially eosinophilic inflammation.

Keyword

B cell-activating factor; Child; Asthma; Sputum

MeSH Terms

Asthma
B-Lymphocytes
Child
Eosinophil Cationic Protein
Eosinophils
Humans
Immunoglobulin E
Inflammation
Methacholine Chloride
Pneumonia
Respiratory Function Tests
Sputum
T-Lymphocytes
Eosinophil Cationic Protein
Immunoglobulin E
Methacholine Chloride