Korean J Pediatr Infect Dis.  2012 Apr;19(1):28-36.

Prevalence of Viruses with Diarrhea among Hospitalized Children West Gyeonggi Province

• Seo SY
• Jung IA
• Kim JH
• Cho KS
• Bin JH
• Kim HH
• Lee HJ
• Lee W

Abstract

PURPOSE
This study was conducted to evaluate epidemiological data of the viral pathogens obtained from stool exams and provide information on the regional prevalence of infectious diarrheal disease west in Gyeonggi Province, Korea.
METHODS
We enrolled a cohort of children <10 years of age admitted for treatment of acute diarrhea at Bucheon St. Mary's Hospital, College of Medicine, The Catholic University of Korea. In total, 310 fecal specimens, documented to be free of common bacterial pathogens, were collected from pediatric patients during a 12-month period from January to December 2009 and were tested for the presence of rotavirus, parechovirus, adenovirus, astrovirus, enterovirus, and norovirus using polymerase chain reaction (PCR) and reverse transcription polymerase chain reaction (RT-PCR) assay.
RESULTS
The most common virus was parechovirus (16%), followed by adenovirus (15%), astrovirus (14%), rotavirus (13%), and enterovirus (5%). Interestingly, only one of the specimens was positive for norovirus. Single infection cases were detected in 173 (55.8%) of the 310 children, whereas mixed viral infections were detected in 10 (3.2%) of the same children. Viral gastroenteritis generally showed a double peak of incidence. Parechovirus, rotavirus, and adenovirus shared a similar pattern of peak incidence with overall viruses; however, astrovirus infections occurred more frequently in the spring. Eighty-five percent of the confirmed viral gastroenteritis cases developed in under 24 months.
CONCLUSION
The results support the importance of parechovirus, adenovirus, astrovirus, and enterovirus as causative agents of diarrhea in children, which may be underestimated by current routine diagnostic testing.

Keyword

Parechovirus; Adenovirus; Astrovirus; Rotavirus; Enterovirus; Norovirus

MeSH Terms

Adenoviridae
Child
Child, Hospitalized
Cohort Studies
Diagnostic Tests, Routine
Diarrhea
Dysentery
Enterovirus
Gastroenteritis
Humans
Incidence
Korea
Norovirus
Parechovirus
Polymerase Chain Reaction
Prevalence
Reverse Transcription
Rotavirus
Viruses

Figure

• 

  Fig. 1 Distribution of rotavirus-, adenovirus-, astrovirus-, norovirus-, enterovirus-, and parechovirus-positive detection by PCR and RT-PCR among 183 stool samples from hospitalized children in Korea. Distribution (%) of the total number of single (n=173) and mixed (n=10) viral intestinal infections among 310 stool samples from hospitalized children. We identified 55.8% (173/310) of single infections and 3.2% (10/310) of multiple infections.

• 

  Fig. 2 Monthly distribution of sporadic gastroenteritis cases during 2009.

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