Effects of Intestinal Ischemia on Intestinal Mucosal Damage and Effects of Interleukin-10 in Young Murine Model

Park, Young Shil; Kim, Dong Chul; Chun, Chung Sik

Abstract

PURPOSE
Intestinal vascular ischemia is the most probable pathogenesis in the development of necrotizing enterocolitis in prematures. The authors studied the histology of intestinal mucosal damage by clamping the superior mesenteric artery in 35 rats, and, analyzed the histologic scores between young rats(I) and adult rats(II) groups according to clamping times(15, 30 and 60 minutes) and compared the effect of interleukin-10(IL-10) in five subjects.
METHODS
The superior mesenteric artery supplying the small intestine was clamped by a small vessel clamp. IL-10 was applied subcutaneously every three days. All rats were killed on the third day after clamping, and were evaluated pathologically by histologic scoring scale adapted from Chiu, et al.
RESULTS
The histologic score of group I was significantly higher than that of group II(P<0.05). When groups I & II were compared in terms of ischemic time, histologic scores showed significant statistical differences in the 60 minute group(9.2+/-1.304, 6.6+/-1.14, respectively, P<0.05). In young rats, the histologic score of the 30 minute group was significantly higher than that of the 15 minute group(P<0.05). Also in adult rats, the histologic score of the 60 minute group was significantly higher than that of the 30 minute group(P<0.05). The histologic score of the IL-10 treated-group was significantly lower than that of the non-treated group(P<0.05).
CONCLUSION
More rapid and severe mucosal damage by ischemia was found in young rats than adult rats. We also expect that IL-10 may have an effect on treatment and prevention of ischemic change, but more extensive study is required.