Clin Exp Otorhinolaryngol.  2014 Dec;7(4):269-274. 10.3342/ceo.2014.7.4.269.

The Expression of AGO2 and DGCR8 in Idiopathic Sudden Sensorineural Hearing Loss

Affiliations
  • 1Department of Otolaryngology, Keimyung University School of Medicine, Daegu, Korea. entnamsi@dsmc.or.kr
  • 2Department of Immunology, Keimyung University School of Medicine, Daegu, Korea.
  • 3Department of Preventive Medicine, Keimyung University School of Medicine, Daegu, Korea.

Abstract


OBJECTIVES
The microRNAs have been implicated in the development and function of the inner ear, especially in contribution to hearing. However, the impact of idiopathic sudden sensorineural hearing loss (SSNHL) on expression of miRNA biogenesis-related components has not been established. To investigate the regulations of microRNA (miRNA) biogenesis-related components, argonaute 2 (AGO2) and DiGeorge syndrome critical region gene 8 (DGCR8) mRNA expression in SSNHL and to evaluate the value of clinical parameters on their expression.
METHODS
Thirty-seven patients diagnosed with SSNHL and fifty-one healthy volunteers were included in this study. We measured mRNA expression levels of AGO2 and DGCR8 in whole blood cells but erythrocytes of patients with SSNHL and controls, using reverse transcription and real-time polymerase chain reaction analysis.
RESULTS
The mRNA expression level of AGO2 is upregulated in SSNHL. The expression level of AGO2 was significantly correlated with that of DGCR8 in both patients with SSNHL and controls. Expression level of AGO2 in SSNHL was correlated with white blood cell counts.
CONCLUSION
This study demonstrated for the first time that the AGO2 mRNA expression level was upregulated in SSNHL, suggesting its important role in pathobiology of SSNHL development.

Keyword

MicroRNA biogenesis; Sudden hearing loss; DiGeorge syndrome critical region gene 8; Argonaute 2

MeSH Terms

Blood Cells
DiGeorge Syndrome
Ear, Inner
Erythrocytes
Healthy Volunteers
Hearing
Hearing Loss, Sensorineural*
Hearing Loss, Sudden
Humans
Leukocyte Count
MicroRNAs
Real-Time Polymerase Chain Reaction
Reverse Transcription
RNA, Messenger
Social Control, Formal
MicroRNAs
RNA, Messenger

Figure

  • Fig. 1 The relative mRNA expression levels of AGO2 and DGCR8 in patients with sudden sensorineural hearing loss and healthy controls (mean±SD). *P<0.05. AGO2, argonaute-2; DGCR8, DiGeorge syndrome critical region gene 8.

  • Fig. 2 Correlation between AGO2 and DGCR8 mRNA expression levels in patients with sudden sensorineural hearing loss and healthy controls. AGO2, argonaute-2; DGCR8, DiGeorge syndrome critical region gene 8.


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