Korean J Otorhinolaryngol-Head Neck Surg.  2010 Sep;53(9):527-533. 10.3342/kjorl-hns.2010.53.9.527.

Expression and Distribution of Tumor Necrosis Factor-Alpha in Mice Cochlea Exposed to Noise

• Jo MH
• Kim CJ
• Koh SH
• Nam GS
• Jeong HM
• Lee JH
• Lee SH

• KMID: 2277157
• DOI: http://doi.org/10.3342/kjorl-hns.2010.53.9.527

Abstract

BACKGROUND AND OBJECTIVES
Recent studies have shown that inflammatory responses occur in the inner ear under various damaging conditions including noise-overstimulation.Identification of time-dependent expression patterns of pro-inflammatory cytokines during the response initiation should lead to rational therapeutic strategies that block the response and reduce the damaging sequelae. MATERIALS AND METHOD: We evaluated the time-dependent expression pattern of pro-inflammatory cytokines in noise-exposed mouse cochlea (white noise, 120 dB SPL, 3 hours) using immunohistochemistry and reverse transcriptase-polymerase chain reaction(RT-PCR).
RESULTS
The most potent pro-inflammatory cytokine, the tumor necrosis factor-alpha (TNF-alpha), was up-regulated after noise exposure. Immunohistochemical analyses showed that the TNF-alpha expression was distinctively induced within the spiral ganglion and stria vascularis. RT-PCR showed that TNF-alpha was induced shortly after noise exposure and persisted upto seven days following noise exposure.
CONCLUSION
Taken together, acoustic trauma induces cochlear inflammation and the data suggest that TNF-alpha may have some role in cochlea damage that occur following noise exposure.

Keyword

Noise-induced hearing loss; Cytokines; Tumor necrosis factor-alpha

MeSH Terms

Animals
Cochlea
Cytokines
Ear, Inner
Hearing Loss, Noise-Induced
Immunohistochemistry
Inflammation
Mice
Noise
Spiral Ganglion
Stria Vascularis
Tumor Necrosis Factor-alpha
Cytokines
Tumor Necrosis Factor-alpha