Detection of Cyclin D1 Protein in Inflammation, Dysplasia and Carcinoma of the Laryngeal Mucosa

Bai, Chang Hoon; Choi, Young Ho; Chen, Seung Min; Kim, Yong Dae; Shim, Young Ran; Song, Si Youn

Abstract

BACKGROUND AND OBJECTIVES: Cyclin D1 is one of the proteins regulating G1-S transition in the cell cycle and is considered to play an important role in subsequent mitotic division. So it is a candidate of a proto-oncogene implicated in the pathogenesis of several human tumor types, including laryngeal squamous cell carcinomas. The purpose of this study was to investigate the development of tumorigenesis and clinicopathologic means of cyclin D1 protein in squamous cell carcinoma of the larynx.
SUBJECTS AND METHOD
Sixty two patients, who have been treated with benign lesion (keratosis and chronic inflammation), dysplasia or squamous cell carcinoma of the larynx from March 1994 to December 1996 were investigated for this purpose. cyclin D1 protein was detected by immunohistochemical technique in the paraffin embedded tissues.
RESULTS
The expression of cyclin D1 protein was detected in 6 out of 11 cases (54.5%) in benign lesion, 3 out of 5 cases (60.0%) in mild to moderate dysplasia, 12 out of 18 cases (66.7%) in severe dysplasia and carcinoma in situ, and 36 out of 44 cases (81.8%) in invasive squamous cell carcinoma of the larynx. Of these, the expression of cyclin D1 protein in invasive squamous cell carcinoma was the highest positive rate, and there was significant difference (p<0.05). However, the expression of cyclin D1 protein was not correlated with degree of differentiation, anatomical site, T-stage and N-stage in invasive squamous cell carcinoma of the larynx (p>0.05).
CONCLUSION
Cyclin D1 protein may be considered to play an important role in the development of the tumorigenesis in invasive squamous cell carcinoma of the larynx.