Korean J Otolaryngol-Head Neck Surg.  2004 Dec;47(12):1289-1296.

Vaccinations with Dendritic Cells Can Elicit Preventive Immunity in a Squamous Cell Carcinoma Animal Model

• Son YI
• Ko YJ
• Chung SW
• Lee CK

Abstract

BACKGROUND AND OBJECTIVES
Dendritic cells (DC) can effectively mediate the prevention and regression of a variety of solid malignancies when they are properly primed with tumor antigens. However, little has been determined about the efficacy of DCbased vaccine for the prevention of squamous cell carcinoma (SCC). SUBJECTS AND METHOD: Bone marrow derived DC of C3H mouse were pulsed with ultraviolet B-irradiated apoptotic SCCVII cells, which are known as a poorly immunogenic SCC cell line. After vaccinations with those DC, a tumorigenic dosage of healthy SCCVII cells were challenged into the flank of animals and the tumor growth was assessed in a blinded and coded fashion. RESULTS: DC pulsed with apoptotic SCCVII cells effectively prevented the formation of solid tumor in 81% of vaccinated animals and mediated significant retardation of tumor growth (p=0.0029) in 19% of vaccinated animals when compared to control groups. SCCVII cell-specific T-cell response (IFN-gamma production) was observed in the immunized mice. CONCLUSION: Adoptive transfer of DC primed with apoptotic SCCVII cells can serve as an effective preventive vaccine in the poorly immunogenic SCC animal model.

Keyword

Dendritic cells; Vaccines; Squamous cell carcinoma; Immunotherapy; Animal models

MeSH Terms

Adoptive Transfer
Animals*
Antigens, Neoplasm
Bone Marrow
Carcinoma, Squamous Cell*
Cell Line
Dendritic Cells*
Immunotherapy
Mice
Mice, Inbred C3H
Models, Animal*
T-Lymphocytes
Vaccination*
Vaccines
Antigens, Neoplasm
Vaccines