Korean J Otolaryngol-Head Neck Surg.
2001 Jul;44(7):736-743.
Experimental studies on the Significance of New MAGE Common Primers Detecting MAGE 1-6 mRNA in Head and Neck Cancers
- Affiliations
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- 1Department of Otolaryngology-Head and Neck Surgery, College of Medicine, Kosin University, Pusan, Korea.
- 2Department of Pathology, College of Medicine, Kosin University, Pusan, Korea.
- 3Department of Clinical Pathology School of Medicine, Catholic University of Taegu, Korea.
- 4Department of Immunology, Keimyung University School of Medicine, Taegu, Korea.
Abstract
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BACKGROUND AND OBJECTIVES: MAGE (melanoma antigen gene) gene, coding for a tumor antigen recognized by cytotoxic T lymphocytes, is expressed specifically in a variety of cancers, but not in normal tissues that exclude those of the testis. Therefore, most studies of MAGE gene are reported for the cancer immunotherapy. There are, however, few reports for the molecular diagnosis of cancers with MAGE gene. The purpose of this study is to evaluate the MAGE common primers in discriminating the malignant from benign lesions of the head and neck.
MATERIALS AND METHODS
Reverse transcriptase-polymerase chain reaction (RT-PCR) and the nested PCR with the MAGE common primers (C1/C2, C3/C4) designed to detect MAGE 1-6 genes in this study were conducted in 37 cancer tissues (27 squamous cell carcinomas and 10 nonsquamous cell carcinoma lesions) and 18 benign tumors and 20 normal tissues of the head and neck . The PCR product was also assayed by RT-PCR with the MAGE 1-6 specific primer (M1-M6) to investigate which MAGE isotypes were detected.
RESULTS
At least more than one isotype of MAGE 1-6 genes were detected in 70.4% (19/27) of squamous cell carcinomas and 30% (3/10) of non-squamous cell carcinoma lesions. But there was no MAGE expression in 18 benign lesions and 20 normal tissues.
CONCLUSION
MAGE common primers designed in this study showed high sensitivity and specificty in discriminating the malignant from benign lesions of the head and neck. This result suggests MAGE gene might be a potential tumor marker in the head and neck cancer.
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