Expression of MAGE 3 Gene Product in Squamous Cell Carcinomas of the Head and Neck

Lee, Kang Dae; Jo, Young Ki; Kim, Sun Gon; Lee, Kil Soo; Choi, Chang Young; Lee, Ju Hong

Abstract

BACKGROUND AND OBJECTIVES: MAGE 3 gene may constitute a potential target for cancer immunotherapy since it is expressed in a variety of cancers but not in normal tissues except the testis. In this study, expression and intracellular location of MAGE 3 gene products were investigated using squamous cell carcinomas of the head and neck.
MATERIALS AND METHODS
MAGE 3 protein expression was screened in 40 squamous cell carcinomas, 2 tumor lines, 20 benign diseases, and 20 normal tissues of the head and neck. Immunohistochemical staining with anti-MAGE 3 mAb 57B was conducted from fresh frozen specimens. A correlation between MAGE 3 expression and clinicopathological parameters was also evaluated.
RESULTS
MAGE 3 gene product was detected in squamous cell carcinomas (18/40, 45%) and in tumor lines (2/2, 100%), but not in benign diseases and normal tissues. MAGE 3 gene product was identified as a cytoplasmic protein of cancer cells without staining in normal epithelia and stromal tissues coexisting adjacent to cancer cells. No significant correlation between MAGE 3 expression and clinicopathological parameters including tumor cell differentiation, age, gender, primary site, tumor stage, and metastasis was drawn.
CONCLUSIONS
MAGE 3 antigen could represent a potential target for immunotherapy in head and neck squamous cell carcinomas.