Korean J Obstet Gynecol.  2003 Dec;46(12):2466-2472.

The Relationship between the Expressions of Common MAGE Gene Productions and the Infection of Human Papillomavirus 16, 18 type in the Cervical Swabs from the Patients of Cervical Neoplasia

• Koh SB
• Choi YS
• Park JB
• Jeon CH
• Lee TS

Abstract

OBJECTIVE
This study was performed to evaluate the expression of MAGE gene and human papillomavirus (HPV) 16, 18 type in patients with cervical neoplasia using in exfoliated cervical cells.
METHODS
The expression of common MAGE gene (MAGE 1 to 6) in cervical scraps was studied by reverse-transcription polymerase chain reaction (RT-PCR) in 12 normal control patient and 48 patients with abnormal cytologic results. In all 60 patients, colposcopic directed biopsy and HPV test by polymerase chain reaction (PCR) was done.
RESULTS
The pathologic results of 60 patients was 12 cervicitis, 37 cervical intraepithelial neoplasia (14 LSIL, 23 HSIL), and 11 invasive squamous cell carcinomas. No expression of common MAGE gene was detected in 12 cervical scraps of cervicitis. The common MAGE gene of LSIL, HSIL and invasive squamous cell carcinomas was expressed in two (14.3%), seventeen (73.9%), ten (90.9%) respectively. HPV type 16 or 18 was detected in 1 cervicitis, 5 LSIL, 20 HSIL and 10 invasive squamous cell carcinoma. In 51 patients (85%), the HPV test result and the common MAGE gene expression showed coincident results.
CONCLUSION
This result suggests common MAGE gene might be potential tumor marker in cervical neoplasia and adjuvant test of the Papanicolaou smear.

Keyword

Common MAGE gene; Exfoliated cervical cells; Human papilloma virus

MeSH Terms

Biopsy
Carcinoma, Squamous Cell
Cervical Intraepithelial Neoplasia
Gene Expression
Human papillomavirus 16*
Humans*
Papanicolaou Test
Polymerase Chain Reaction
Uterine Cervicitis