Korean J Otolaryngol-Head Neck Surg.  2000 Jul;43(7):751-757.

Angiogenesis and p53 Mutation in Oral and Oropharyngeal Squamous Cell Carcinoma

• Sun DI
• Kim MS
• Cho SH
• Kim BK
• Suh BD

Abstract

BACKGROUND AND OBJECTIVES: It is well known that microvessel density (MVD) and p53 gene mutation are significantly correlated with tumor behaviors in some types of cancer: however, some studies have reported a lack of relationship among MVD, p53 gene mutation and tumor behavior in oral cancers. The objective of this study was to identify putative association between p53 gene mutation and microvessel density (MVD) and to evaluate the usefulness of this association in deciding the therapeutic plan. MATERIALS AND METHODS: In 25 tumor specimens of oral squarnous cell carcinoma, microvessel density (MVD) was analysed by immunohistorhemical staining with CD-31 monoclonal antibody, and p53 mutation was examined in exon 5 through 8 by PCR-SSCP and sequencing analysis.
RESULTS
Seven of the 25 patients had mutation in exon 5 to 8 and all the mutations were missense mutation. The mean of MVD in the mutant group was 13.3+/-2.80 and that of MVD in the wild type group was 18.6+/-1.16. An inverse relationship was seen between MVD and p53 mutation (p=0.047). The p53 gene mutation was frequently found in exon 5.
CONCLUSION
MVD and p53 gene mutation were not associated with respect to stage, cervical metastasis and recurrence of' the oral and oropharyngeal cancer. Angiogenesis of oral squamous cell carcinoma might not be regulated by p53, but might be regulated by other factors.

Keyword

Angiogenesis; MVD; p53 mutation; Oral and oropharyngeal squamous cell carcinoma

MeSH Terms

Carcinoma, Squamous Cell*
Exons
Genes, p53
Humans
Microvessels
Mouth Neoplasms
Mutation, Missense
Neoplasm Metastasis
Oropharyngeal Neoplasms
Recurrence