Korean J Orthod.  2012 Jun;42(3):118-128. 10.4041/kjod.2012.42.3.118.

Histopathologic investigation of the effects of prostaglandin E2 administered by different methods on tooth movement and bone metabolism

• Caglaroglu M
• Erdem A

• KMID: 2272260
• DOI: http://doi.org/10.4041/kjod.2012.42.3.118

Abstract

OBJECTIVE
The aim of this study was to investigate and compare the in vivo effects of prostaglandin E2 (PGE2) administered by different methods on orthodontic tooth movement and bone metabolism macroscopically, histopatologically, and biochemically.
METHODS
Forty-five young adult New Zealand rabbits were randomly divided into 3 experimental groups (n = 10/group), 1 positive control group (n = 10), and 1 negative control group (n = 5). The experimental rabbits were fitted with springs exerting 20-g reciprocal force on the maxillary incisors and PGE2 (10 microg/mL) was administered by the intravenous, submucosal, or intraligamentous route after appliance insertion and on days 1, 3, 7, and 14 thereafter. All rabbits were sacrificed on day 21 and their premaxillae were resected for histologic evaluation.
RESULTS
Tooth movement was observed in the experimental and positive control groups, but the intraligamentous PGE2 group had the highest values of all analyzed parameters, including serum calcium and phosphorus levels and osteoclastic and osteoblastic populations (p < 0.001).
CONCLUSIONS
Submucosal and intraligamentous PGE2 administration significantly increases orthodontic tooth movement and bone metabolism, but the intraligamentous route seems to be more effective.

Keyword

PGE2; Experimental tooth movement; Intraligamentary; Optical dissector method

MeSH Terms

Calcium
Dinoprostone
Humans
Incisor
Osteoblasts
Osteoclasts
Phosphorus
Rabbits
Tooth
Tooth Movement
Young Adult
Calcium
Dinoprostone
Phosphorus

Figure

• 

  Figure 1 The experimental appliance. Appearance of the spring (A), its insertion (B), and the subsequent tooth movement (C).

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  Figure 2 Hematoxylin and eosin-stained sample from the negative control group. Magnification: left, ×2.5; right, ×63. D, Dentin; PDL, periodontal ligament; AB, alveolar bone; O, osteocyte.

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  Figure 3 Hematoxylin and eosin-stained sample from the positive control group. The pressure (A, B) and tension (C, D) sides are shown. Magnification: A and C, ×2.5; B and D, ×63. D, Dentin; PDL, periodontal ligament; AB, alveolar bone; OB, osteoblast; OC, osteoclast; RF, resorption foci.

• 

  Figure 4 Hematoxylin and eosin-stained sample from the intravenous PGE2 group. The pressure (A, B) and tension (C, D) sides are shown. Magnification: A and C, ×2.5; B and D, ×63. D, Dentin; PDL, periodontal ligament; AB, alveolar bone; OB, osteoblast; OC, osteoclast; RF, resorption foci; MPS, median palatine suture.

• 

  Figure 5 Hematoxylin and eosin-stained sample from the submucosal PGE2 group. The pressure (A, B) and tension (C, D) sides are shown. Magnification: A and C, ×2.5; B and D, ×63. D, Dentin; PDL, periodontal ligament; AB, alveolar bone; OB, osteoblast; OC, osteoclast; RF, resorption foci; C, capillary.

• 

  Figure 6 Hematoxylin and eosin-stained sample from the intraligamentous PGE2 group. The pressure (A, B) and tension (C, D) sides are shown. Magnification: A and C, ×2.5; B and D, ×63. D, Dentin; PDL, periodontal ligament; AB, alveolar bone; OB, osteoblast; OC, osteoclast; RF, resorption foci; MPS, median palatine suture; C, capillary.

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