Korean J Obstet Gynecol.  2003 Nov;46(11):2216-2220.

Studies on the Effects of Monoclonal Antibodies to Mammalian Heat Shock Protein 60 (HSP 60) on Mouse Embryo Development In Vitro

• Lee IH
• Chung KN
• Kim YB

Abstract

OBJECTIVE
To investigate the effects of antibodies to HSP 60 on the early mouse embryo development in vitro.
METHODS
The 175 late 2-cell mouse embryos were obtained from 6-7 week old female ICR mice. 5-10 embryos were placed in each well. The embryos were incubated in the Ham's F-10 medium supplemented with 100 microgram/mL of monoclonal antibody to HSP 60 (66), monoclonal mouse IgG1 (55), and medium alone (54), respectively, at 37degrees C in a 5% CO2 humidified air chamber, and mouse embryo developments were observed daily.
RESULTS
On day4, growth arrests were more prominent in anti-HSP 60 containing group compared to IgG1 containing group, medium only group (0% vs 16%, 14%), and these results were statistically significant (p=0.0032). Especially those inhibitory effects were observed in early stage of embryo development (day1) and these results were also statistically significant (31% vs 83%, 77%, p<0.0001). Moreover, we found out that cellular degenerations were more common in anti-HSP 60 containing group and this features were prominent on day2.
CONCLUSION
Anti-HSP 60 elicited a strong growth inhibitory and degenerative effect on early mouse embryo development. These findings suggest that HSP 60 may exert a protective effect against mouse embryo degeneration or apoptosis.

Keyword

Heat shock protein; Monoclonal antibody; Embryo development

MeSH Terms

Animals
Antibodies
Antibodies, Monoclonal*
Apoptosis
Chaperonin 60*
Embryonic Development*
Embryonic Structures*
Female
Heat-Shock Proteins*
Hot Temperature*
Humans
Immunoglobulin G
Mice*
Mice, Inbred ICR
Pregnancy
Antibodies
Antibodies, Monoclonal
Chaperonin 60
Heat-Shock Proteins
Immunoglobulin G