A histologic study of initial change and repair of tooth and periodontal tissue in extrusion of young adult dogs

Yoon, Byung Sun; Cha, Kyung Suk; Lee, Jin Woo

Abstract

This study was carried out in order to study early histologic changes and repair reaction appling to extrusive force for 3rd premolar of adult dogs. After 1 week of extrusive force with elastic chain, one of dogs was sacrified and after 3 weeks retention period, another dog was sacrified. The paraffin sections of samples were stained with Hematoxylin - Eosin and Masson's Trichrome and were examed by light microscopy . The obtained results as follows. 1, In Hematoxylin - Eosin and Masson Trichrome stain of control group , the periodontal ligament width was constant from apical third to cervical third of the root and periodontal fiber arrangement was horizontal or oblique in cervical third, oblique in middle third, oblique in apical third of root. in alveolar bone, smooth appearance was shown. 2. In Group 1, all periodontal fiber arrangement was oblique toward tooth, and the periodontal ligament width increased . Partially PDL was ruptured in apex. In MT stain, immature bone formation was seen at alveolar crest area. Active bone formation was observed along the one side of alveolus, and apical portion of pulp was involved with blood vessel rupture , vacuolization of pulp tissue and hyperemia. 3. In Group 2, most periodontal ligament arrangement and PDL width was repaired and fiber density increased In MT stain, mineralization of immatuie bone on the alveolar crest was progressed. In pulp, vacuole and hyperemia was diminished and fibrotic change was diminished. 4. After 3 week periodontal ligament has more repair ability than pulp tissue. pulp was involved with vacuolization and fibrosis, so it takes more time for repair.