Korean J Nutr.  2005 Jun;38(5):364-372.

Effects of Vitamin E and Dehydroepiandrosterone on The Formation of Preneoplastic Lesions in Rat Hepatocellular Carcinogenesis

• Kim S
• Choi H

Abstract

This study is designed to examine the effects of dietary supplementation with vitamin E and dehydroepiandrosterone (DHEA) on the formation of preneoplastic lesions in diethylnitrosamine (DEN) induced rat hepatocarcinogenesis. All Weaning male Sprague-Dawley rats were initiated by a single dose of DEN (200 mg/kg body weight), subjected to twothirds partial hepatectomy 3 weeks later and were sacrificed 8 weeks after DEN initiation. Two weeks after initiation, rats were fed Purina purified rodent diet 5053 (Ralston Purina Rat chow, USA) with 1.5% (15,000 IU/kg diet) vitamin E, 0.5% DHEA and both of those supplemented diet for 6 weeks. Placental glutathione S-transferase (GST-P) positive foci, the activities of catalase, total-glutathione peroxidase (GPx), glutathione reductase (GR), glutathione S-transferase (GST) and thiobarbituric acid reactive substances (TBARS) contents were decreased significantly by vitaimin E supplement. On the other hand GST-P positive foci number, Cu/Zn-superoxide dismutase (SOD) and glucose 6-phosphatase (G6Pase) activities weren't changed by vitamin E supplement. It might suggest that protective effect of vitamin E against hepatocarcinogens is not involved in the formation of the GST-P positive foci but related to the expansion of that. It seemed that vitamin E supplement helped endogenous defense system in carcinogenesis by decreasing TBARS contents, H2O2, organic peroxides. Therefore, vitamin E seemed to protect cell from free radical damage in carcinogenesis . By DHEA supplement liver weight and liver/body ratio were increased, the area and number of GST-P positive foci, the activities of catalase, GR, total GPx, GST and the TBA RS contents were decreased significantly. On the other hand Cu/Zn-SOD and G6Pase activities weren't changed by DHEA supplement. In hepatocarcinogenesis the activities of antioxidant enzymes weren't increased by DHEA supplement. DHEA did not increase the oxidative stress, while DHEA seems to have anticarcinogenic effect in rats hepatocarcinogenesis.

Keyword

hepatocarcinogenesis; vitamin E; dehydroepiandrosterone; GST-P positive foci; antioxidant enzymes

MeSH Terms

Animals
Anticarcinogenic Agents
Carcinogenesis*
Catalase
Dehydroepiandrosterone*
Diet
Dietary Supplements
Diethylnitrosamine
Glucose-6-Phosphatase
Glutathione Reductase
Glutathione Transferase
Hand
Hepatectomy
Humans
Liver
Male
Oxidative Stress
Peroxidase
Peroxides
Rats*
Rats, Sprague-Dawley
Rodentia
Thiobarbituric Acid Reactive Substances
Vitamin E*
Vitamins*
Weaning
Anticarcinogenic Agents
Catalase
Dehydroepiandrosterone
Diethylnitrosamine
Glucose-6-Phosphatase
Glutathione Reductase
Glutathione Transferase
Peroxidase
Peroxides
Thiobarbituric Acid Reactive Substances
Vitamin E
Vitamins