Korean J Nutr.  2012 Oct;45(5):437-442. 10.4163/kjn.2012.45.5.437.

The effect of medicinal herb administration to arthritis inducded rabbit arthritistreatment

• Kwon TD
• Kim KH

• KMID: 2268738
• DOI: http://doi.org/10.4163/kjn.2012.45.5.437

Abstract

The purpose of this study was is to investigate the arthritis treatment and prevention of the effects the herbral administration to experimental animals induces arthritis 4 weeks for medicinal herbs (Scutellaria baicalensis Georgi, Acanthopanacis cortex, Achyranthis Radix). To investigate the prevention of histopathological examination and molecular biological examinations for arthritis improvement in vivo New Zealand, white rabbits were divided into a normal (Normal, n = 7), or a collagen-induced arthritis (CIA, n = 7), or a collagenase injection and medicinal herbs (SAA, n = 7). It was confirmed that induced arthritis was treated with Gross examination, and the measurement of average arthritis index (MAI) and improved arthritis by medicinal herbs. TNF-alpha and IL-1beta were significantly increased in the CIA and SAA groups, compared to the normal group (p < 0.05). MMP-1 and TIMP-2 were significantly increased in the CIA, compared to the other groups (p < 0.05). MMP-1 and TIMP-2 were significantly decreased in the SAA group, compared to the CIA group (p < 0.05). As a result, Herbal administration used in this study might be able to help in the treatment of arthritis induced by inflammatory and immunosuppressive effects, that which can be expected.

Keyword

medicinal herbs; arthritis; inflammatory

MeSH Terms

Animals
Arthritis
Arthritis, Experimental
Collagenases
Humans
New Zealand
Plants, Medicinal
Rabbits
Tissue Inhibitor of Metalloproteinase-2
Tumor Necrosis Factor-alpha
Collagenases
Tissue Inhibitor of Metalloproteinase-2
Tumor Necrosis Factor-alpha

Figure

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  Fig. 1 The collagenase injectioned knee of rabbit (arrow) was more swollen compared with the other knee.

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  Fig. 2 Immunohistochmical stain of knee joints (TB protocol, ×400).

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  Fig. 3 The concentration of TNF-α (A) and IL-1β (B) in the blood of rabbits after 4 weeks treatment of each samples. a: p < .05 vs. normal.

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  Fig. 4 The concentration of MMP-1 (A) and TIMP-2 (B) in the synovial fluids of Rabbits after 4 weeks treatment of each samples. a: p < .05 vs. Normal, b: p < .05 vs. CIA.

Reference

1. Quinn MA, Green MJ, Marzo-Ortega H, Proudman S, Karim Z, Wakefield RJ, Conaghan PG, Emery P. Prognostic factors in a large cohort of patients with early undifferentiated inflammatory arthritis after application of a structured management protocol. Arthritis Rheum. 2003. 48(11):3039–3045.

2. Visser H, le Cessie S, Vos K, Breedveld FC, Hazes JM. How to diagnose rheumatoid arthritis early: a prediction model for persistent (erosive) arthritis. Arthritis Rheum. 2002. 46(2):357–365.

3. Lard LR, Visser H, Speyer I, vander Horst-Bruinsma IE, Zwinderman AH, Breedveld FC, Hazes JM. Early versus delayed treatment in patients with recent-onset rheumatoid arthritis: Comparisoncomparison of two cohorts who received different treatment strategies. Am J Med. 2001. 111(6):446–451.

4. Jung SM, Schumacher HR, Kim H, Kim M, Lee SH, Pessler F. Reduction of urate crystal-induced inflammation by root extracts from traditional oriental medicinal plants: Elevationelevation of prostaglandin D2 levels. Arthritis Res Ther. 2007. 9(4):R64.

5. Park KC, Park EJ, Kim ER, Kim Y, Chung SH, Cho BW, Kim S, Jin M. Therapeutic effects of PG201, an ethanol extract from herbs, through cartilage protection on collagenase-induced arthritis in rabbits. Biochem Biophys Res Commun. 2005. 331(4):1469–1477.

6. Shin SS, Jin M, Jung HJ, Kim B, Jeon H, Choi JJ, Kim JM, Cho BW, Chung SH, Lee YW, Song YW, Kim S. Suppressive effects of PG201, an ethanol extract from herbs, on collagen-induced arthritis in mice. Rheumatology (Oxford). 2003. 42(5):665–672.

7. Gao XY, Wang DW, Li FM. Determination of ecdysterone in Achyranthes Bidentatabidentata Bl. and its activity promoting proliferation of osteoblast-like cells. Yao Xue Xue Bao. 2000. 35(11):868–870.

8. Goldring SR, Gravallese EM. Pathogenesis of boneerosionsbone erosions in rheumatoid arthritis. Curr Opin Rheumatol. 2000. 12(3):195–199.

9. Salinardi BJ, Roush JK, Schermerhorn T, Mitchell KE. Matrix metalloproteinase and tissue inhibitor of metalloproteinase in serum and synovial fluid of osteoarthritic dogs. Vet Comp Orthop Traumatol. 2006. 19(1):49–55.

10. Giannelli G, Erriquez R, Iannone F, Marinosci F, Lapadula G, Antonaci S. MMP-2, MMP-9, TIMP-1 and TIMP-2 levels in patients with rheumatoid arthritis and psoriatic arthritis. Clin Exp Rheumatol. 2004. 22(3):335–338.

11. Huh JE, Baek YH, Kim YJ, Lee JD, Choi DY, Park DS. Protective effects of butanol fraction from Betula platyphyla var. japonica on cartilage alterations in a rabbit collagenase-induced osteoarthritis. J Ethnopharmacol. 2009. 123(3):515–521.

12. Ekenstedt KJ, Sonntag WE, Loeser RF, Lindgren BR, Carlson CS. Effects of chronic growth hormone and insulin-like growth factor 1 deficiency on osteoarthritis severity in rat knee joints. Arthritis Rheum. 2006. 54(12):3850–3858.

13. Bezerra MC, Carvalho JF, Prokopowitsch AS, Pereira RM. RANK, RANKL and osteoprotegerin in arthritic bone loss. Braz J Med Biol Res. 2005. 38(2):161–170.

14. Christodoulou C, Choy EH. Joint inflammation and cytokine inhibition in rheumatoid arthritis. Clin Exp Med. 2006. 6(1):13–19.

15. Smolen JS, Aletaha D, Koeller M, Weisman MH, Emery P. New therapies for treatment of rheumatoid arthritis. Lancet. 2007. 370(9602):1861–1874.

16. Choy E. Clinical experience with inhibition of interleukin interleukin-6. Rheum CisDis Clin North Am. 2004. 30(2):405–415.

17. Andreakos ET, Foxwell BM, Brennan FM, Maini RN, Feldmann M. Cytokines and anti-cytokine biologicals in autoimmunity: present and future. Cytokine Growth Factor Rev. 2002. 13(4-5):299–313.

18. Cole P, Rabasseda X. The soluble tumor necrosis factor receptor etanercept: a new strategy for the treatment of autoimmune rheumatic disease. Drugs of Today Today (Barc). 2004. 40(4):281–324.

19. Eijsbouts AM, Kempers MJ, van den Hoogen FH, Laan RF, Hermus AR, Ross HA, Sweep FC, van de Putte LB. Effects of naproxen and sulphasalazine or methotrexate on hypothalamic-pituitary-adrenal axis activity in patients with rheumatoid arthritis. Clin Exp Rheumatol. 2011. 29(1):35–42.

20. Francoz D, Desrochers A, Simard N, Saint Pierre Y, Fecteau G, Latouche JS, Fortin M. Relative expression of matrix metalloproteinase-2 and -9 in synovial fluid from healthy calves and calves with experimentally induced septic arthritis. Am J Vet Res. 2008. 69(8):1022–1028.

21. Heppner KJ, Matrisian LM, Jensen RA, Rodgers WH. Expression of most matrix metalloproteinase family members in breast cancer represents a tumor-induced host response. Am J Pathol. 1996. 149(1):273–282.

22. Murphy G. Tissue inhibitors of metalloproteinases. Genome Biol. 2011. 12(11):233.

23. Visse R, Nagase H. Matrix metalloproteinases and tissue inhibitors of metalloproteinases: Structurestructure, function, and biochemistry. Circ Res. 2003. 92:827–839.