Korean J Nutr.  2011 Dec;44(6):465-473. 10.4163/kjn.2011.44.6.465.

Anti-atherosclerotic Effect of Green Tea in Poluynsaturated Fatty Acids-treated Apo E KO Mice

• Kim HS
• Lee M

• KMID: 2268606
• DOI: http://doi.org/10.4163/kjn.2011.44.6.465

Abstract

Dietary fatty acids are under intense research to identify anti-atherogenic mechanisms, so we investigated green tea powder (GT) as a protector against atherogenesis originating from lipid peroxidation such as 4-hydroxynonemal (4-HNE) and malondialdehyde (MDA) in different dietary fatty acid-treated apo E KO mice. Growth rate and dietary efficiency were lower in apo E KO mice with or without LA compared to wild type. Plasma total cholesterol (TC) and triacylglycerol (TG) did not correspond to values in other tissues, but TG in heart tissue decreased significantly by GT after linoleic acid (LA) or docosahexaenoic acid (DHA) was administered. LA induced apoptosis as evidenced by changes in aorta morphology and immunohistochemistry. Lipid peroxides (LPO) was increased in apo E KO mice with or without LA corresponding to the accumulation of 4-HNE or MDA in the proximal aorta above the atria. GT consumption tended to reduce the primary causal mechanism of atherogenic phenomena such as oxidizability in both LA and DHA treated atherogenic mice. A high polyunsaturated fatty acids (PUFA) diet involved the changes on stress-induced apoptotic signaling by increasing caspase 3, cytochrome c, and nuclear factor-kappaB in the heart tissue, but decreasing the bcl-2 protein. However, GT remarkably reduced the expression of apoptotic signaling, in contrast to the PUFA diet. Therefore, the potential of GT as an anti-atherosclerotic dietary antioxidant was tested in this study.

Keyword

green tea; antiatherosclerosis; 4-HNE; aorta morphology; apoptosis

MeSH Terms

Animals
Aorta
Apolipoproteins E
Apoptosis
Atherosclerosis
Caspase 3
Cholesterol
Cytochromes c
Diet
Fatty Acids
Fatty Acids, Unsaturated
Heart
Immunohistochemistry
Linoleic Acid
Lipid Peroxidation
Lipid Peroxides
Malondialdehyde
Mice
Plasma
Tea
Triglycerides
Apolipoproteins E
Caspase 3
Cholesterol
Cytochromes c
Fatty Acids
Fatty Acids, Unsaturated
Linoleic Acid
Lipid Peroxides
Malondialdehyde
Tea
Triglycerides

Figure

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  Fig. 1 Research design for this study.

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  Fig. 2 A: Gross morphology of proximal aortae in 20%LA, 20%LA + 5%GT, 20%DHA and 20%DHA + 5%GT treated apo E KO mice. The presence of atherosclerotic lesion (white arrow) were clearly observed at inner-curvature and branch points of aorta in apo E KO mice. B: Representative image of an aortic root atherosclerotic lesion (white arrow) in LA, LA-GT, DHA and DHA-GT treated C57BL/6J apoE KO mice.

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  Fig. 3 Cell signaling of mitochondrial damage from the hepatocytes of apoE KO mice and 20% LA, 20% LA + 5% GT, 20% DHA and 20% DHA + 5% GT treated apo E KO mice.

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